摘要
采用免疫组化 S- P法研究表皮生长因子受体 (EGFR)、增殖细胞核抗原 (PCNA)在口腔粘膜上皮异型增生及口腔鳞癌组织中的表达意义及其相互关系。结果表明 ,EGFR及 PCNA在正常口腔粘膜上皮为阴性或仅在上皮基底层有少量阳性表达。上皮异型增生时 ,随病变程度加重 ,阳性表达呈递增趋势 (P<0 .0 1) ,至重度异型增生时 ,PCNA表达与鳞癌无显著性差异 (P>0 .0 5 ) ,EGFR表达甚至超过高分化鳞癌。口腔鳞癌组织随分化程度降低 ,阳性表达率相应增加 (P<0 .0 1)。EGFR表达与 PCNA表达有明显相关性 (P<0 .0 1)。 EGFR和 PCNA可作为评估和监测口腔粘膜上皮恶变潜能 ,判断口腔鳞癌恶性度的有用标记物。
Using S- P immunohistochemical method to study the significance of expression and relationship between epidermal growth factor receptor( EGFR) and proliferating cell nuclear antigen ( PCNA) in dysplasia of oral mucosal epithelium and squamous cell carcinoma.The results showed that EGFR and PCNA immunostaining were negative in normal epithelium and there were only a few positive cells limited to the basal layer.In dysplasis,as the lesions were progressing,the positive expression accordingly increased( P<0 .0 1 ) .The expression of PCNA had no significant difference between severe dysplasia and carcinoma( P>0 .0 5) .The rate of EGFR expression in severe dysplasia was higher than that in well- differentiated carcinoma.As the differentiated degree of carcinoma was decreasing,the positive rate of EGFR and PCNA increased in oral squamous cell carcinoma( P<0 .0 1 ) .There was a close correlation between EGFR and PCNA in expression( P<0 .0 1 ) .EGFR and PCNA may be served as a useful marker for predicting the malignant change of oral mucosa and determining the malignant degree of carcinona.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2000年第3期308-311,共4页
Chinese Journal of Histochemistry and Cytochemistry
基金
安徽省教委科研基金资助课题!(97J10 82 )
