摘要
目的 :探讨致敏后重复抗原攻击大鼠肺组织 c GMP- PDE、c AMP- PDE活性变化。方法 :HPL C法测定致敏后重复抗原攻击的大鼠 (抗原攻击组 )、致敏不攻击的大鼠 (致敏组 )和正常大鼠 (正常组 )肺组织 c GMP- PDE、c AMP- PDE活性。结果 :抗原攻击组肺组织 c GMP- PDE、c AMP- PDE活性分别为 798.76± 5 2 9.2 3、5 97.5 0± 2 2 0 .75nmol·g-1protein·min-1,其中抗原攻击组大鼠肺组织 c GMP- PDE活性较正常组明显升高 (P<0 .0 5 ) ,抗原攻击组大鼠肺组织 c AMP- PDE活性较致敏组和正常组均明显升高 (P<0 .0 5 )。结论 :大鼠哮喘发病过程中肺组织c GMP- PDE、c AMP-
Objective: To assess the change of cGMP PDE and cAMP PDE activity in a rat lung model of asthma. Methods: cGMP PDE and cAMP PDE activity were determined by HPLC. Results: Both cAMP PDE activity and cGMP PDE activity in the lung tissue of antigen challenged rats were higher than that from the normal rats( P <0.05). Conclusion: In the rat lung model of asthma, cyclic nucleotides phosphodiesterase activity was elevated. This may be significant in the pathogenesis of asthma.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2002年第2期127-130,共4页
Journal of Zhejiang University(Medical Sciences)
基金
国家自然科学基金资助项目 (39970 85 7)
