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GA调节禾谷类α-淀粉酶基因表达的信号转导及分子机制 被引量:19

Physiological and Molecular Mechanisms of GA Regulating α-Amylases Gene Expression in Cereal Grains
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摘要 用GAs处理禾谷类糊粉细胞原生质体后 ,可以诱导α_淀粉酶的合成与分泌。ABA抑制GAs的诱导作用并可刺激ABA诱导蛋白的产生。GAs和ABA的受体位于质膜上。最近的研究表明 :G蛋白、cGMP、Ca2 +和钙调素、三磷酸肌醇 (IP3)及蛋白质磷酸酶 (PP1和PP2A)都不同程度的参与了GA响应的信号传导过程。已克隆出一些可在转录水平上调节GA诱导基因的顺反子 ,并证明它们在禾谷类糊粉细胞中的GA响应事件中起至关重要的作用。有证据表明GA在α 淀粉酶的转录后水平的调节上也有作用。 The synthesis and secretion of α_amylases was induced by gibberellins (GAs) in the protoplasts of cereal aleurone layer cells.ABA repressed the effects of GA and stimulated the production of ABA_up_regulated proteins. Receptors for GA and ABA had been localized on the aleurone cell PM. Recent researches proved that G_protein,cGMP,Ca 2+ and CaM,IP 3 and protein phosphatases 1 and 2A were involved in the signal transduction pathways of GA response event. Some cis_acting elements and trans_action factors which regulate GA_induced genes at the transcription level were cloned. They played an important role in the GA response event of the cereal aleurone cells. Some evidences showed that GA had effects on the post_transcription level of α_amylases.
出处 《植物学通报》 CAS CSCD 北大核心 2002年第1期63-69,共7页 Chinese Bulletin of Botany
基金 广东省自然科学基金团队项目资助 ( 0 0 30 6 2 )
关键词 调节 分子机制 GAs 禾谷类 Α-淀粉酶 基因表达 信号转导 GA, Cereal, α_amylases, Geen expression, Signal transduction
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  • 1Menchi Chang,Baiyang Wang,Xiongfong Chen,Ray Wu. Molecular characterization of catalytic-subunit cDNA sequences encoding protein phosphatases 1 and 2A and study of their roles in the gibberellin-dependent Osamy-c expression in rice[J] 1999,Plant Molecular Biology(1):105~115 被引量:1
  • 2Richard Hooley,Michael H. Beale,Sally J. Smith. Gibberellin perception at the plasma membrane of Avena fatua aleurone protoplasts[J] 1991,Planta(2):274~280 被引量:1

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