摘要
[目的]探讨HSV TK基因在膀胱癌动物体内的合适转移途径 ,观察HSV TK/GCV系统的体内治疗效果。[方法]在T739同基因鼠膀胱癌皮下肿瘤模型中 ,以裸质粒DNA瘤内直接注射、脂质体包裹和逆转录病毒介导3种不同方法转移HyTK基因 ,观察GCV对膀胱癌的治疗效果。[结果]脂质体复合物组及逆转录病毒组背部肿瘤生长减慢 ,抑瘤率分别为54%、68%。动物平均存活时间分别比对照组延长28.81%、44.16%。[结论]脂质体。
To investigate effective HSV-TK gene transfer methods on bladder cancer in vivo and observe the effects of HSV-TK/GCV system.In vivo, effects on T739 syngeneic mice bladder cancer were observed by direct gene transfer with naked DNA,DNA-liposome complex and retroviral-mediated gene therapy.Tumor growth inhibit rate was 54% and 68% in group DNA-liposome complex and retroviral-mediated gene transfer respectively, animal survival time was prolonged with 28.81% and 44.16% increasing respectively than the control group.[Conclusion]DNA-liposome complex and retroviral-mediated gene transfer are effective methods for bladder cancer gene therapy.
出处
《肿瘤学杂志》
CAS
2002年第2期81-83,共3页
Journal of Chinese Oncology
基金
重庆市科委基金资助
