摘要
目的 探讨不同剂量氯磷定和阿托品联合呼吸机治疗氧化乐果中毒所致呼吸肌麻痹的疗效。方法 所有实验大鼠均给予 2LD50 的氧化乐果染毒 ,并以 10mg/kg的阿托品有效对抗胆碱能症状。当大鼠出现呼吸频率减慢 ,呼吸困难征象时即行气管插管并辅助机械通气 (A组除外 ) ,B组阿托品继续原剂量治疗 ,C、D、E组氯磷定依次按 15、2 0、4 0mg/kg于呼吸机治疗即刻及治疗后 1、2、3h肌肉注射 ,阿托品减至首剂量的 1/3～ 2 /3,以维持阿托品化为度。经联合治疗后 1、2、3h试行脱机 ,若大鼠在上述时间中任何一次脱机 >6 0min ,则视为联合治疗成功。一次脱机后大鼠存活超过 6 0min或第 3次脱机后迅速死亡 ,均需取游离膈神经膈肌标本经MS 30 2生理药理分析仪作膈肌功能测定。结果 B组膈肌功能恢复不佳 ,无一只大鼠脱机成功 ;C组膈肌功能恢复良好 ,3h脱机成功率高达10 0 % ;D、E两组虽膈肌功能测定也较好 ,但脱机成功率极低。结论 只有适量氯磷定联合阿托品并辅助呼吸机治疗氧化乐果中毒所致的呼吸肌麻痹 ,才能加速中毒大鼠膈肌功能恢复 。
Objective To examine the therapeutic effect of combined use of pralidoxime Cl and atropine with artificial ventilation on respiratory muscle paralysis caused by omethoate poisoning in rats. Methods Rats were administered with same doses of 2LD 50 omethoate and then treated with atropine (10 mg/kg) to resist effectively chlolinergic symptoms. When the rats had slow respiratory frequency and breathed with difficulty, the trachea was intubated and artificial ventilation was carried out (except for group A ) . The rats in group B were continuously treated with atropine. The doses of pralidoxime Cl for group C,D and E were 15 mg/kg,20 mg/kg and 40 mg/kg respectively,given at the same time as artificial ventilation and 1,2 and 3 hours later. The dose of atropine was reduced to 1/3 to 2/3 of the first dose so as to maintain the rats atropinized. If the rat survived beyond 60 minutes after withdrawal of artificial ventilation,the combined treatment was considered successful. The function of isolated phrenic diaphragm of the rats was observed with MS 302 analyses instrument physiologically and pharmacologically.Results None of the rats in group B successfully withdraw from artificial ventilation. The rats in group C all successfully withdraw from artificial ventilation in 3 hours and the function of the isolated phrenic muscle remained good. The survival rats in group D and E were very low after withdrawal ,even though the function of isolated phrenic muscle was good . Conclusions The therapeutic effect of the combined use of suitable dose of pralidoxime Cl and atropine with artificial ventilation on respiratory muscle paralysis caused by omethoate poisoning in rats was significant. This measure can facilitate reversal of the function of poisoned diaphragm and reduced the death rate in poisoned rats.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2002年第4期259-261,共3页
Chinese Journal of Internal Medicine
