摘要
将拟南芥茉莉素受体基因COI1在水稻中的同源基因OsCOI1a构建到植物双元表达载体pMYC2,导入拟南芥茉莉素受体突变体coi1-1中,采用Western blot检测OsCOI1a基因的蛋白表达水平,通过观察突变体花粉的活性和果荚的育性,检测OsCOI1a对coi1-1突变体的互补情况,研究OsCOI1a基因的功能。结果表明,OsCOI1a基因能够在拟南芥coi1-1突变体中正确表达,并能部分互补雄性不育的表型,表明OsCOI1a基因在水稻茉莉素信号通路中可能起茉莉素受体作用。
We cloned OsCOI1a into the expression vector pMYC2,and transformed it into Arabidopsiscoi1-1 mutants. We tested the protein expression levels by western blot,observed the activity of pollen and the sterility of siliques in coi1-1 mutants transformed with OsCOI1 a,and found out that OsCOI1 a could partly complement the phenotype of the sterility of Arabidopsis coi1-1 mutants. All these results suggested that OsCOI1 a might be a receptor of jasmonates in rice.
出处
《作物研究》
2014年第4期337-340,共4页
Crop Research
基金
国家自然科学基金项目(30770195)
