摘要
目的 建立原发性胆汁性肝硬化 (PBC)特异性免疫学检测方法。方法 在重组质粒表达的基础上 ,用亲和层析进一步纯化重组蛋白后 ,用酶免疫吸附法检测M2抗体。结果 在PBC组 1 1例患者中全部检出M2抗体 ,阳性率为 1 0 0 % ,而非PBC组 75例患者中无一检出M2抗体。本法与病理检查和临床诊断的相关性有非常显著意义 (P <0 .0 1 )。结论 本法检测M2抗体有较好的敏感性及特异性 。
Objective For establishment and application of enzyme immune assay to detect M2 antibody with BCOADC, PDC, OGDC, BPO as antigen. Methods After expressing autoantigens of primary biliary cirrhosis (PBC) in prokaryote by using of constructing recombinant expressive plasimid successfully, the fusion protein was purified by affinity chromatography. M2 antibody was tested in PBC patients and non PBC patients using enzyme immune assay. Results M2 antibody was 100%(11/11) positive in PBC patients and negative of non patients. Conclusion The detection system showed good sensitivity and specificity.It may be used as a powerful and sepecific method for the diagnosis of PBC.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2002年第2期75-77,共3页
Chinese Journal of Laboratory Medicine
基金
国家自然科学基金 ( 30 0 80 0 2 7
39570 6 73)
上海市百人计划基金资助项目 ( 9713)
