摘要
用聚合酶链反应 (PCR)方法扩增 86例恶性血液病患者 P1 6 及 P1 5基因的外显子 1及外显子 2 ,再用限制性内切酶— PCR方法检测两基因的甲基化。发现 2 9例患者有 P1 6 、P1 5基因失活 ,以甲基化失活为主。对 P1 6 、P1 5基因失活与甲基化进行相关性分析 ,发现 P1 6 、P1 5基因失活与甲基化呈正相关 ,且患者的治疗效果差、缓解率低、缓解期明显缩短。认为 P1 6及 P1 5基因失活和甲基化的检测对于探讨恶性血液病的发病机制。
Using PCR method to investigate the extron land 2 of P 16 and P 15 gene in 86 cases with malignant hematological diseases,the correlation of gene delation and methylation was studied Restriction enzyme PCR technique detected gene methylation Results showed methylation was major pathway of P 15 and P 16 gene inactivation Devitalization of P 15 and P 16 gene had positive correlation Patients with P 15 and P 16 gene inactivation had a poor response to chemotherapy and a low remmission This suggested inactivation of P 15 and P 16 gene played an important role in the pathogenesis of malignant diseases
出处
《山东医药》
CAS
北大核心
2002年第6期10-11,共2页
Shandong Medical Journal
