摘要
目的 探讨刺五加 (AS)注射液对脑缺血性损伤的保护作用及量效关系 .方法 4 0只雄性 SD大鼠 ,随机分为四组(各组 n=10 ) ,即对照组和 AS- 2 .5 ,AS- 5 ,AS- 7.5组 ,分别在缺血前腹腔注射生理盐水 7.5 m L和刺五加注射液 2 .5 m L ,5 .0 m L(均用生理盐水稀释至 7.5 m L) ,7.5 m L.采用颈内动脉尼龙线线栓法致大脑中动脉栓塞 (12 0 min)模型 ,观察再灌注后 2 4 h时神经功能损害并取大脑行 TTC染色以测量脑梗死容积 .结果 再灌注 2 4 h时神经功能损害评分 ,对照组为(2 .2± 0 .8) ,明显高于各保护组 :AS- 2 .5组 (1.3± 0 .8)、AS-5 .0组 (1.2± 0 .8)、AS- 7.5组 (1.2± 0 .6 ) ,P<0 .0 5 .脑梗死容积对照组为 (2 2 0± 6 6 ) mm3,明显大于各保护组 [AS- 2 .5组为 (116± 86 ) mm3,AS- 5 .0组为 (12 1± 79) mm3,AS- 7.5组为(10 4± 5 4 ) mm3],P<0 .0 5 .各保护组之间神经功能损害评分和脑梗死容积无明显差别 .
AIM To investigate the protective effect of acanthopanax senticosus (AS) injection, an intravenous drug made from traditional Chinese herb, on transient focal cerebral ischemic injury in rats. METHODS Forty male SD rats were randomly divided into 4 groups: Control group ( n =10)received no pharmacologic intervention but normal saline 7.5 mL, Group AS 2.5 ( n =10), AS 5 ( n =10), AS 7.5 ( n =10) received intraperitoneally acanthopanax senticosus injection 2.5 mL, 5 mL (diluted to 7.5 mL with normal saline) and 7.5 mL respectively just before middle cerebral artery occlusion (MCAO) with 3 0 nylon monofilament for 120 min. The neurological outcome was evaluated at 24 h after reperfusion. The infarct volume was then assessed by TTC staining. RESULTS The neurologic deficit score (NDS) of Group AS 2.5 (1.3±0.8), Group AS 5 (1.2±0.8) and Group AS 7.5 (1.2±0.6) at 24 h after reperfusion was lower than that of the Control group (2.2±0.8), P <0.05. The infarct volume of the Control group (220±66) mm 3 was significantly larger than those of the other three groups [AS 2.5: (116±86) mm 3, AS 5.0: (121±79) mm 3, AS 7.5: (104±54) mm 3] at 24 h after reperfusion. There was no difference among AS treated groups for NDS and infarct volume. CONCLUSION Acanthopanax senticosus injection significantly reduces neurologic injury induced by transient MCAO without dose response effect.
出处
《第四军医大学学报》
北大核心
2002年第8期698-700,共3页
Journal of the Fourth Military Medical University
