摘要
将固定好的脑组织样品 ,按常规方法制备成石蜡切片。切片脱蜡入水 ,用蛋白酶K消化去除正常PrP蛋白 ,留下异常PrP蛋白。然后用适当工作浓度的兔抗牛PrP血清孵育切片 ,再用DAKOChemMateTM检测试剂盒处理。同时结合H·E染色法检测我国疯牛病。实验发现 ,在1131例国内样品中免疫组化的实验结果均为阴性 ,H·E染色的结果均未发现有特征性海绵状空泡变化。实验进一步证实 ,免疫组织化学方法是一种操作简单、结果容易判断、价格低廉的检测手段 。
Paraffin sections were prepared by routine method from fixed brain tissue samples dewaxed and placed in water. The normal PrP proteins were removed by protase K digestion and the abnormal PrP proteins were left. Sections were incubated with rabbit anti-bovine PrP serum and treated then with DAKO ChemMate kit for detection of BSE in China in combination with H·E staining. It was found that samples of 1131 cases all had negative results in immunohistochemical assay, and had negative results in immunohistochemical assay and no characteristic spongy vacuole changes. Tests confirmed that immunohistochemical assay was a simple, easily readable and low-cost method, suitable for BSE detection in China.
出处
《中国动物检疫》
CAS
2002年第4期25-27,共3页
China Animal Health Inspection
基金
农业部948项目
农业部疯牛病监测项目
青岛市科技发展计划项目 (2001-2)资助
