摘要
为了鉴定所构建的鸡下丘脑组织 c DNA文库能否用于下丘脑表达谱的建立 ,从 c DNA文库中随机挑取 12个克隆 ,对其表达序列标签 (expressed sequence tags,ESTs)进行了测定和初步分析。经 BL ASTn和 FASTA3.1分析后发现 ,1个 ESTs在 Gen Bank中可以找到鸡的同源序列 ,4个在其他物种中也可以找到同源序列 ,1个在 ESTs database中有同源 ESTs序列 ,还有 6个为未知功能基因。 12个 ESTs序列均已录入 Gen Bank。
In order to monitor quality of the chicken hypothalamus cDNA library constructed and whether it could be used to construct the expression profile of hypothalamus,we sequenced and primarily analyzed 12 ESTs from randomly selected clones.By BLASTn and FASTA analysis in GenBank,1 EST could find homology in chicken,4 had matching sequences in turkey or other species,1 had homologous EST sequence in EST database,6 were function unknown gene.All 12 ESTs sequence were loaded into GenBank.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2002年第2期184-186,共3页
Chinese Journal of Veterinary Science
基金
国家基础研究重点发展规划项目 ( G2 0 0 0 0 16 10 0 )
国家杰出青年基金资助项目 ( 3972 5 0 2 2 )
关键词
鸡
下丘脑
CDNA文库
表达序列标签
chicken
hypothalamus
cDNA library
expressed sequence tags(ESTs)
