摘要
利用在大肠杆菌中表达的藻红蓝蛋白 α-亚基脱辅基蛋白与藻蓝胆素 PCB重组 ,吸收光谱、荧光光谱和高效可逆光化学性质分析表明 ,藻红蓝蛋白 α-亚基脱辅基蛋白与藻蓝胆素直接重组 ,生成的胆素蛋白中辅基色素仍为藻蓝胆素 ;而藻红蓝蛋白 α-亚基脱辅基蛋白与藻蓝胆素在藻红蓝蛋白 α-亚基重组酶 (pec E和 pec F基因的表达产物 )催化下重组 ,生成的胆素蛋白中辅基色素转变为藻紫胆素 ,并具有高效可逆光化学特性。
Using the expressed and renatured apo-α-phycoerythrocyanin to be reconstituted with phycocyanobilin, the prosthetic group in the reconstitued phycobiliproteins were analyzed with absorption spectra. When apo-α-phycoerythrocyanin was reconstituted with phycocyanobilin directly, the prosthetic group in the produced phycobiliprotein is still phycocyanobilin. However, when apo-α-phycoerythrocyanin was reconstituted with phycocyanobilin under catalysis of α-phycoerythrocyanin lyase, the prosthetic group in the produced phycobiliprotein is phycoviolobilin, which possessed the highly reversible photochemistry property.
出处
《武汉植物学研究》
CAS
CSCD
2002年第1期38-42,共5页
Journal of Wuhan Botanical Research
基金
国家自然科学基金 (编号 :39770 175 )
武汉市晨光计划 (编号 :96 5 0 0 1037-2 1)资助项目
