摘要
目的 探讨反义胰岛素样生长因子Ⅱ (反义IGF -Ⅱ )对人肝癌细胞株HepG2 恶性表型的抑制。方法 化学合成人IGF -Ⅱ 5’端部分cDNA片段 ,将其克隆于真核表达载体pcDNA3 ,通过脂质体介导转入体外培养的HepG2 人肝癌细胞 ,观察细胞生物学特性的改变。结果 将反义IGF -Ⅱ转导入HepG2 肝癌细胞后 ,细胞内IGF -Ⅱ表达量减少 (荧光强度由 19.9± 3.1降至 6 .2± 1.8,(P <0 .0 1) ,细胞增殖率下降、凋亡率增加 (由 4 .3%± 0 .5 0 %增加至 8.6 %± 0 .5 0 % ,(P <0 .0 1)、AFP分泌水平降低 (由5 .4 7μg/l± 0 .5 6 μg/l降至 1.95 μg/l± 0 .10 μg/l,(P <0 .0 1)。 结论 反义IGF -Ⅱ可显著抑制人肝癌细胞恶性表型 ,对于治疗原发性肝癌可能具有潜在应用价值。
Objective Effect of antisense insulin like growth factor Ⅱ(IGF Ⅱ) on biological character of human hepatocellular carcinoma cell line HepG 2 was studied. Methods A cDNA in 5' of human IGF Ⅱ was synthesized and inserted into an eukaryotic expression vector of pcDNA 3 , and was introduced into human hepatocellular carcinoma cell line HepG 2 by liposomes.The change of cells' biological character was observed. Results Of HepG 2 cells transfected with antisense IGF Ⅱ, the expression of IGF Ⅱ reduced (from 19.9±3.1 to 6.2±1.8), the proliferation was lowered, the apoptosis rate increased(from 4.3%±0.5% to 8.6%± 0.50%), the secretation of AFP reduced(from 5.47μg/l±0.56μg/l to 1.95μg/l±0.10μg/l ). Conclusion Antisense IGF Ⅱ has inhibition effects on malignant phenotype of HepG 2 cells and may provide an effective approach to the treatment of human hepatoma.
出处
《肿瘤》
CAS
CSCD
北大核心
2002年第2期104-106,共3页
Tumor
基金
广东省自然科学基金资助 (940 3 19)
关键词
反义胰岛素样生长因子Ⅱ
肝癌细胞
恶性表型
HEPG2
Antisense
Insulin like growth factor Ⅱ(IGF Ⅱ)
Hepatocellular carcinoma cell
Malignant phenotype
