摘要
目的 建立幽门螺杆菌 (Helicobacterpylori,Hp)蒙古沙鼠感染模型 ,并通过比较定量培养、快速脲酶试验和ELISA三种方法 ,确定Hp动物模型的最佳评判指标。方法 采用临床分离的Hp强毒株对 2 0只蒙古沙鼠进行感染 ,4周后剖杀。取血按常规ELISA法检测抗Hp抗体 ;分别剪取胃窦、胃体和胃底粘膜组织作定量培养 ;其余组织作快速脲酶试验及病理检查。结果 经定量培养结果显示沙鼠的Hp感染率达到 80 % (16/ 2 0 ) ,胃窦、胃体和胃底的Hp定植密度的对数均值分别为 5 2 4± 1 5 0、4 11± 3 2 2、和0 97± 2 3 9;16只Hp阳性沙鼠中脲酶试验有 4只阳性 ,ELISA有 7只阳性 ,阳性鼠的感染均值显著高于阴性鼠。结论 经筛选的Hp强毒株成功感染沙鼠后 4周 ,Hp主要定植于胃窦和胃体部 ;
Objective To establish a Mongolian gerbil model infected with H. pylori and to determine its most suitable index by the means of quantitative culture,urease test and ELISA. Methods Twenty Mongolian gerbils were orally administered with H. pylori . Four weeks after inoculation, all animals were killed with dissection. H. pylori infection index were evaluated by quantitative culture and urease test of gastric tissue and the serum H. pylori specific antibodies were measured with ELISA method. Results Eighity percent of gerbils(16/20) were infected. The density of colonization in the gastric antrum, body of and fundus of stomach, expressed in the form of lg CFU/g, was 5.24±1.50,4.11±3.22 and 0.97±2.39, respectively. Of 16 gerbils stomach with H. pylori colonization in quantitative culture, 12 were negative in urease test and 9 were negative in ELISA. Conclusion H. pylori colonizes predominantly in gastric antrum and body of stomach in Mongolian gerbils 4 weeks after inoculation. For assessment of H. pylori colonization in the gerbil model, quantitative culture is the choice.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2002年第3期286-288,共3页
Journal of Third Military Medical University
基金
国家重点科技攻关计划项目( 96 - 90 1- 0 1- 54 )
关键词
幽门螺杆菌
动物模型
定量培养
蒙古沙鼠
Helicobacter pylori
animal model
Mongolian gerbil
quantitative culture
