摘要
目的探讨SCD1基因缺失对小鼠脏器参数,血液生理生化指标的影响。方法选取8周龄SCD1^(-/-)小鼠及其杂合子SCD1^(+/-)小鼠,称取体质量及主要脏器,观察其脏器系数。比较不同周龄SCD1^(-/-)小鼠及其杂合子SCD1^(+/-)小鼠的血液细胞学和生化指标,观察其变化特点。结果 8周龄SCD1^(-/-)小鼠与杂合子小鼠比较肝脏系数显著升高(P<0.05)。6~8周龄SCD1^(-/-)小鼠与杂合子小鼠的血液细胞学指标相比RBC、MPV、PCT显著降低(P<0.05),MCV、MCH、RDW、PDW、PLT、LYM显著增高(P<0.05);血清生化指标ALB、TC、TG极显著降低(P<0.01),ALP、ALT、UA极显著增高(P<0.01)。16~18周龄SCD1^(-/-)小鼠与杂合子小鼠的血液细胞学指标MCHC、PLT、PCT相比极显著降低(P<0.01),RDW、HCT、MCV都极显著增高(P<0.01);血清生化指标ALP、TC极显著降低(P<0.01)。结论 SCD1基因缺失对小鼠鼠脏器参数、血液生理生化指标都有一定的影响。
Objective To investigate the effect of SCD1 gene deletion on organ coefficients and hematology indexes of mice. Method Organ coefficients are observed in 8 weeks SCD1^-/- mice and heterozygous SCD1 mice as well as hematology indexes of different ages mice are compared between the two group. Result Liver coefficient of 6-8 weeks SCD1^-/-mice markedly increase compared with heterozygous mice. RBC,MPV,PCT of SCD1^-/-mice are significantly lower(P<0.05),while MCV,MCH,RDW,PDW,PLT,LYM are remarkbly higher(P<0.05) than those of the control group. ALB,TC,TG also obviously go down and ALP,ALT,UA clearly go up(P<0.01)in 6-8 weeks experiment group. MCHC, PLT, PCT,ALP, TC of 16-18 weeks significantly decrease compared with those of SCD1^+/-mice. At the same time,RDW、HCT and MCV significantly increases(P<0.01). Conclusion SCD1 gene deletion impairs the organ coefficients of mice. Moreover, gene SCD1 deletion has impact on some hematology indexes.
作者
朱祥宇
陈烨
王学文
田枫
陈章健
周淑佩
ZHU Xiangyu;CHEN Ye;WANG Xuewen;TIAN Feng;CHEN Zhangjian;ZHOU Shupei(Peking University People's Hospital,Beijing 100044,China;The Department of Laboratory Animal Science,Health Science Center,Peking University ,Beijing 100191,China;School of public Health,Peking University ,Beijing 100191,China)
出处
《实验动物科学》
2018年第6期22-27,共6页
Laboratory Animal Science
基金
北京大学医学部优博培育计划建设项目(No.BMU20160564)