摘要
目的以痰热清注射液中处方成分金银花为对象,通过鉴定其金银花蛋白,建立质谱法测定蛋白多肽的方法,探索中药注射液使用质谱检测蛋白的可能性。方法考察三氯乙酸(TCA)/丙酮沉淀法、苯酚抽提两种方法的蛋白提取效果,采用UPLC/Q-TOF-MS/MS法分析鉴定金银花药材中的蛋白,并针对所测得的蛋白建立三重四级杆检测方法,以多反应监测模式对痰热清注射液及中间体中的金银花蛋白进行检测。结果金银花水提液中检测到核酮糖-1,5-二磷酸羧化酶、F1-ATP酶α亚单位、翻译延伸因子1-α部分等3种蛋白,注射液中未检出蛋白,方法检测限为1μg·m L^(-1)。结论使用质谱法目标性测定痰热清注射液中金银花特征蛋白,为中药注射液蛋白测定提供新的解决思路。
Objective The mass-spectrographic method was established to determine the peptides and proteins of Lonicera japonica flos which was the main ingredient of Tanreqing injection.Methods TCA/Acetone precipitation and phenol extraction were assessed for protein extraction;and UPLC/Q-TOF-MS/MS method was established to detect the protein and analyze peptides of intermediates and Tanreqing injections.Results Three proteins were detected in the intermediates and no protein was in the injections with the detection limit of 1 μg·m L^-1.Conclusion The mass-spectrography was applied to detect protein from Lonicera japonica flos in Tanreqing injection objectively,which was a new solution for protein detection in TCM injections.
作者
张甦
陈丹丹
于泓
胡青
季申
ZHANG Su;CHEN Dandan;YU Hong;HU Qing;JI Shen(Shanghai Institute of Food and Drug Control,Shanghai 201203 Shanghai,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2018年第1期61-66,共6页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
上海市卫生和计划生育委员会中医药科研基金(2014YP001A)
国家中药标准化项目(ZYBZH-C-SH-48)
上海科委技术服务平台项目(14DZ2294000)
