摘要
目的:发现外周血中与冠状动脉粥样硬化性心脏病(冠心病)相关的未知长链非编码RNA(LncRNA)。方法:选取临床冠心病组及对照组各3例外周血血浆样本进行LncRNA高通量测序表达谱分析,使用cuffdiff软件获得LncRNA表达谱,计算两组样本间的倍数变化和P-value值,筛选差异表达LncRNA。Q-PCR法验证36对冠心病和正常组外周血浆及外周血单核细胞中的基因表达水平。结果:测序结果分析发现差异表达明显的45个上调表达及29个下调表达的LncRNA。Q-PCR法筛选出3个在冠心病患者外周血单核细胞中上调表达明显的LncRNA:uc003pxg.1,ENST00000565257,ENST00000568324。ROC曲线下面积分别为0.812 5,0.742 4,0.742 4。以上基因在冠心病患者中上调表达均差异显著,与测序结果一致。结论:初步从冠心病患者外周血样本中筛选出上调表达的LncRNA,这为后续深入研究奠定基础,为LncRNA用于冠心病临床早期筛查提供理论依据。
Objective:To discover unknown LncRNAs associated with Coronary Artery Disease(CAD)in peripheral blood.Methods:Three pairs of clinical CAD group and control blood plasma samples were texted in this experiment by high-throughput sequencing methods. Cuffdiff software was used to get the expression profiles of LncRNAs,differentially expressed LncRNAs were identified based on fold change and p-value. Differently expressed genes in peripheral plasma and blood monocytes were verified by QPCR in 36 pairs of clinical CAD group and controls. Results:The optimal 45 up-regulated and 29 down-regulated LncRNAs were detected. Three LncRNAs uc003 pxg.1,ENST00000565257,ENST00000568324 were up-regulated obviously in peripheral blood mononuclear cells of CAD patients. Area under the ROC curve results of uc003 pxg.1,ENST00000565257,ENST00000568324 were0.812 5,0.742 4,0.742 4 respectively. These genes were all up-regulated in CAD patients obviously and these outcomes were consistent with the sequencing results. Conclusion:The ideal LncRNAs were detected preliminarily. This founding lays the foundation for further research,and provides theoretical basis of LncRNA using for CAD early clinical screening.
作者
李平
徐桂冬
庞智
翁嘉懿
尹娟
孙康云
Li Ping;Xu Guidong;Pang Zhi;Weng Jiayi;Yin Juan;Sun Kangyun(Central Lab,Affiliated Suzhou Hospital,NMU,Suzhou 215008,China;Department of Cardiology,Affiliated Suzhou Hospital,NMU,Suzhou 215008,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第12期1696-1700,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
苏州市卫生和计划生育委员会临床重点病种诊疗技术专项(LCZX201610)
关键词
冠心病
长链非编码RNA
高通量测序
外周血单核细胞
coronary artery disease
LncRNAs
high-throughput sequencing
peripheral blood mononuclear cells
