摘要
目的:对齿瓣石豆兰的化学成分进行分离及鉴定。方法:将齿瓣石豆兰全草的95%乙醇提取物经不同极性溶剂(氯仿、甲醇)依次萃取,萃取部位再经硅胶柱层析、薄层层析、Sephadex LH-20柱层析和重结晶等方法进行分离纯化,根据化合物的理化性质和光谱数据利用IR、Ms、~1 H-NMR、^(13) C-NMR和HMBC等方法确定其化学结构。结果:从各萃取部位中分离鉴定出了3个黄酮类化合物,分别为化合物6-甲氧基-2-(4'-甲氧基苯基)-3-羟基-色原酮、6,7-二羟基-3-(4'-羟基)异黄酮、化合物5。此外,还分离得到2个其他类型的化合物,分别为对羟基苯甲醛、水杨酸。以上化合物均为首次从齿瓣石豆兰植物中分离得到。其中化合物5为新化合物,命名为黄酮YN,为首次报道。结论:研究结果进一步明确了齿瓣石豆兰的化学成分,可为其药理活性物质基础研究提供参考依据。
Objective:To separate and identify the chemical constituents of Bulbophylluml.evineiSchltr.Methods:95%ethanol extracts from Bulbophylluml.evineiSchltr.wereextractedwithdifferent polar solvents as aether petroleum and ethyl acetate in turn.The extracts parts were isolated and purified by various chromatographic methods including silica gel.ODS.Sephadex LH-20.The chemical structure of them were identified according to physicochemical property and spectral data by IR,Ms,1 H-NMR,13C-NMR and HMBC.Results:Three flavonoids compounds and two the other compounds isolated from 95%ethanol extracts were identified.All of the compounds were isolated from Bulbophylluml.evineiSchltr.firsttime,and the compound 5 was the first report that is a new compound,named flavonoid YN.Conclusion:The study further confirms the chemical constituents of Bulbophyllum.levinei Schltr,and can provide scientific evidence for material base of pharmacological activity of Pholidota cantonensis.
作者
黄丽芸
陈小兵
许军
凌志杰
钟禹霖
彭红
Huang Liyun;Chen Xiaobing;Xu Jun;Ling Zhijie;Zhong Yulin;Peng Hong(Gannan Health Vocational College,Ganzhou 341000,China;Jiangxi University of Traditional Chinese Medieine,Nanchang 330004,China)
出处
《亚太传统医药》
2019年第1期41-44,共4页
Asia-Pacific Traditional Medicine
基金
江西省卫生计生委中医药科研基金(2016B122)
关键词
齿瓣石豆兰
黄酮类成分
分离
萃取
鉴定
Bulbophyllum.Levinei Schltr.
Flavonoid Constituents
Separations
Extraction
Identification
