摘要
多囊卵巢综合征是育龄期妇女常见的代谢紊乱性疾病.前期研究表明,益生菌的干预可以有效改善肠道菌群并缓解以肥胖和2型糖尿病为代表的代谢类疾病,而多囊卵巢综合征患者的临床指标及肠道菌群能否通过益生菌的干预得到改善则有待证实.本研究通过联合使用多组学技术揭示了多囊卵巢综合征患者食用4周益生菌Bifidobacterium lactis V9后肠道微生物组发生的变化.结果表明,Bifidobacterium lactis V9在患者肠道中具有稳定的定殖能力,4周干预期后,患者肠道菌群结构和代谢均发生显著变化,Clostridium,Collinsella,Coprococcus和Klebsiella菌属的丰度显著降低,而Faecalibacterium和Lactobacillus菌属的丰度显著增加.基于代谢组学的分析结果表明,糖皮质激素代谢水平显著降低,乙酸、丙酸和丁酸等短链脂肪酸和琥珀酸的代谢水平显著提高.通过PICRUSt功能预测显示,PPAR信号通路与脂肪细胞因子信号通路显著下降,而基于差异性菌属与代谢功能单元的网络互作分析进一步显示了Bifidobacterium lactis V9对多囊卵巢综合征患者肠道微生物组的调节作用.因此,以肠道微生物为靶点,通过益生菌Bifidobacterium lactis V9干预可以有效调节多囊卵巢综合征患者的肠道微生物组,而这些微生物指标的改善将有助于患者临床症状的缓解,为益生菌治疗代谢类疾病提供更加客观详实的数据支撑.
Polycystic ovary syndrome(PCOS) is a common metabolic disorders among women of reproductive age worldwide. It is also one of the common causes of female infertility. The effect of probiotics has been gradually revealed and recognized by the public. Previous studies have shown that probiotic intervention can effectively improve intestinal microbiome and relieve metabolic diseases represented by obesity and type 2 diabetes. Although a few studies have investigated the intestinal microbiota profiles of women with PCOS, the regulatory effect of the probiotic Bifidobacterium lactis V9 has yet to be revealed. We hope that Bifidobacterium lactis V9 can improve the metabolism of patients by regulating the intestinal flora, thus slowing down the disease. To address this gap, in this study, metagenomics and metabolomics were used together to analyze the effects of Bifidobacterium lactis V9 on patients with PCOS. Nine patients with PCOS were selected to comsume 15 billion probiotic Bifidobacterium lactis V9 every day during the intervention period. Fecal samples were collected from patients at week 0, week 1, week 2 and week 4. The results showed that Bifidobacterium lactis V9 had stable colonization ability in the intestinal tract of patients. The abundance of Bifidobacterium and Faecalibacterium significantly increased, while that of other bacteria significantly decreased, including Clostridium, Collinsella, Ochrobactrum, Streptococcus, Prevotella, Coprococcus and Actinomyces and the opportunistic pathogen Klebsiella is almost gone. The results of metabonomic analysis showed that the metabolic level of glucocorticoids was significantly reduced and the metabolic level of short chain fatty acids such as acetic acid, propionic acid and butyric acid was significantly increased. PPAR signal pathway and adipocytokine signal pathway associated with insulin resistance and lipid metabolism were decreased significantly based on PICRUSt. Furthermore, the regulation functions of Bifidobacterium lactis V9 on intestinal microbiome in p
作者
马臣臣
彭倩楠
姜帅铭
陈开宁
方育团
张家超
Chenchen Ma;Qiannan Peng;Shuaiming Jiang;Kaining Chen;Yuman Fang;Jiachao Zhang(College of Food Science and Technology,Hainan University,Haikou 570228,China;Department of Endocrinology,Hainan Provincial People's Hospital,Haikou 570228,China)
出处
《科学通报》
EI
CAS
CSCD
北大核心
2019年第3期360-368,共9页
Chinese Science Bulletin
基金
科拓恒通益生菌科研青年基金(2017-2)
海南大学科研启动基金(KYQD1548)资助
