摘要
目的通过GEO公共数据库,探索AEBP1高表达组与低表达组在膀胱癌患者临床病理特征的表现差异,判断AEBP1的表达对膀胱癌术后患者特异性生存率与总体生存率的影响。方法对从NCBI中收集到的膀胱癌公共数据集进行回顾性与预后分析,再利用基因集富集分析方法分析AEBP1推动膀胱癌发展的可能机制。结果显示在不同侵袭性(P<0.001)、分级(P<0.001)、T分期(P<0.001)、N分期(P=0.002)的膀胱癌患者中,AEBP1的表达均有显著性差异,具体表现为高表达组临床特征差于低表达组;并且有AEBP1的表达与膀胱癌患者术后的总体生存和特异性生存相关联,AEBP1高表达组膀胱癌患者的肿瘤特异性生存期明显差于AEBP1低表达组膀胱癌患者(P=0.0003,HR=0.272,95%CI:0.135~0.548),AEBP1高表达组膀胱癌患者的总体生存期亦明显差于AEBP1低表达组膀胱癌(P=0.0159,HR=0.5479,95%CI:0.3360~0.8934)。AEBP1高表达样本富集了与肌细胞生成、炎症反应、紫外线反应、MYC信号通路、内皮-间质转换、Notch信号通路、KRAS信号通路、IL6-JAK-STAT通路、IL2-STAT5信号通路、Hedgehog通路、TNF-α通路、补体、干扰素γ、顶端结、凝固、黏液、缺氧、血管生成、顶面、同种异体移植排斥相关的基因。结论AEBP1与膀胱癌中多个病理指标相关联,高表达组的膀胱癌患者临床预后更差,进一步分析AEBP1推动膀胱癌发展的机制,对于膀胱癌的抑制发展和治疗有着重要的意义。
Objective To clarify its relationship with clinical pathological characteristics of bladder cancer, so as to evaluate the function of AEBP1 as a prognosis marker in bladder cancer, AEBP1 is predicted to promote the development of bladder cancer. Methods Retrospective and prognostic analysis of the public data set of bladder cancer collected from NCBI was performed. Gene set analysis method (GSEA) was used to analyze the related gene set regulated by AEBP1. Results The results showed that the expression of AEBP1 was significantly different in bladder cancer patients with different invasive (P<0.001), grade (P<0.001), T stage (P<0.001) and N stage (P=0.002). The expression of AEBP1 was correlated with the overall survival and specific survival of bladder cancer patients. The specific survival time of patients with bladder cancer with high expression of AEBP1 was significantly lower than that of patients with bladder cancer (P=0.0003, HR=0.2723, 95%CI: 0.1353-0.5481), and the overall survival of patients with bladder cancer with high expression of AEBP1 was significantly lower than that of AEBP1 low expression group (P=0.0159, HR=0.5479, 95%CI: 0.3360-0.8934). The expression of AEBP1 was enriched with myosin production, inflammatory response, UV response, MYC signaling pathway, endothelial-stromal transformation, Notch signaling pathway, ILS-JAK-STAT pathway, IL2-STAT5 signaling pathway, Hedgehog pathway, TNF-α pathway, complement, interferon gamma, top node, coagulation, mucus, hypoxia, angiogenesis, top, allograft rejection related gene. Conclusions AEBP1 is associated with multiple pathological indicators in bladder cancer. The prognosis of patients with bladder cancer in high expression group is worse, and prediction can be used as a potential marker for prognosis of patients with bladder cancer and the target of bladder tumor.
作者
蒋佳志
高毓
李胜
王刚
陈松
邓巧玲
Jiang Jiazhi;Gao Yu;Li Sheng;Wang Gang;Chen Song;Deng Qiaoling(Central South Hospital of Wuhan University/second Clinical College of Wuhan University,Wuhan 430071,China;Biological Sample Database of Central South Hospital of Wuhan University,Wuhan 430071,China;Department of Urology,Central South Hospital,Wuhan University,Wuhan 430071, China;Department of Laboratory ,Central South Hospital,Wuhan University,Wuhan 430071,China)
出处
《国际泌尿系统杂志》
2019年第1期17-20,共4页
International Journal of Urology and Nephrology
基金
武汉大学珞珈青年学者科研基金(351人才计划)
武汉大学中南医院科技创新培育基金(znpy2017050).
关键词
膀胱肿瘤
预后
Urinary Bladder Neoplasms
Prognosis
