摘要
目的观察DR5、DcR2在多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠子宫内膜中的表达,探讨PCOS子宫内膜功能失常的其他可能机制。方法大鼠皮下注射硫酸普拉睾酮钠构建PCOS模型,分别用免疫组织化学法、蛋白质免疫印迹分析观察并比较DR5、DcR2在PCOS组和对照组大鼠子宫内的表达。结果免疫组织化学结果显示,DR5和DcR2主要表达于子宫内膜腺上皮细胞,DR5在PCOS组和对照组大鼠子宫内膜腺上皮细胞中的相对表达量分别为0.083±0.004、0.161±0.002,差异有统计学意义(P=0.000);DcR2在两组大鼠子宫内膜腺上皮细胞的相对表达量为0.061±0.003、0.062±0.002,差异无统计学意义(P=0.720)。蛋白质免疫印迹分析显示,DR5在PCOS组和对照组大鼠子宫中相对表达量分别为0.997±0.021、1.282±0.015,差异有统计学意义(P=0.000);DcR2在PCOS组和对照组大鼠子宫中相对表达量分别为0.906±0.078、0.860±0.064,差异无统计学意义(P=0.474)。结论 PCOS大鼠子宫内膜功能发生失常可能与DR5在子宫内膜中的表达下调有关。
Objective To observe the expression of DR5 and DcR2 in the endometrium of polycystic ovary syndrome(PCOS)rats and explore other possible mechanisms of the dysfunction of endometrium in PCOS.Methods Rats were subcutaneously injected with sodium proprteone sulfate to construct the PCOS model.The expression of DR5 and DcR2 in the uterus of PCOS and control rats was compared by immunohistochemistry and western blot respectively.Results Immunohistochemistry assay showed that DR5 and DcR2 were mainly expressed in the endometrium epithelial cells.The relative expression of DR5 in endometrial glandular epithelial cells of PCOS group and control group were 0.083±0.004 and 0.161±0.002 respectively,with statistically significant difference(P=0.000).The relative expression of DcR2 in the endometrial glandular epithelial cells of the two groups were 0.061±0.003 and 0.062±0.002(P=0.720).Western blot assay showed that the relative expression of DR5 in the uterus of PCOS group and control group were 0.997±0.021 and 1.282±0.015 respectively,and the difference was statistically significant(P=0.000).The relative expression of DcR2 in the uterus of PCOS group and control group were 0.906±0.078 and 0.860±0.064,respectively,without significant difference(P=0.474).Conclusion The dysfunction of endometrial function in PCOS may be related to the downregulation of DR5 expression in the endometrium.
作者
王立群
张娟
丁桂清
周月希
WANG Liqun;ZHANG Juan;DING Guiqing;ZHOU Yuexi(First Clinical College of Hubei University of Traditional Chinese Medicine, Wuhan 430070, Hubei Province, China;Reproductive Center, General Hospital of Central Theater Command, Wuhan 430070, Hubei Province, China)
出处
《解放军医学院学报》
CAS
2018年第12期1089-1092,1119,共5页
Academic Journal of Chinese PLA Medical School
基金
湖北省自然科学基金项目(2018CFC806)
武汉市科技局应用基础研究项目(2016060101010030)~~
