摘要
以灵芝为试验材料,采用干质量法测定生物量,苯酚-硫酸法测定胞外多糖产量,研究了单色LED光照处理对灵芝菌丝体液体发酵合成胞外多糖的影响。结果表明:在80μmol·m^(-2)·s^(-1) LED光照12h条件下,不同LED光照处理均能促进细胞生长和胞外多糖合成,其中白光LED最有利于细胞生长,蓝光LED最有利于胞外多糖合成。综合考虑生物量和胞外多糖产量,以100μmol·m^(-2)·s^(-1)蓝光LED光照10h效果最佳,生物量和胞外多糖产量分别为15.16g·L^(-1)和3.102g·L^(-1),比黑暗处理提高了45.91%和109.74%。光照对灵芝细胞生长和胞外多糖合成具有调控作用,为灵芝液体发酵合成胞外多糖的高效定向生产提供指导。
The effects of different LED light treatment on exopolysaccharide synthesis in the liquid fermentation of Ganoderma lucidum were studied.The biomass and the yield of exopolysaccharide were determined by dry weight method and phenol-sulfuric acid method,respectively.The results showed that under the condition of light treatment for 12 hours with intensity of 80μmol·m^-2·s^-1,different LED light treatments could promote cell growth and exopolysaccharide synthesis.White LED light was most conducive to cell growth and blue LED light was best for the synthesis of exopolysaccharides.Taking into account the biomass and the yield of exopolysaccharide,blue LED light treatment for 10 hours with intensity of 100μmol·m^-2·s^-1 had the best effect,the biomass and the yield of exopolysaccharide was 15.16g·L^-1 and 3.102g·L^-1,respectively,which was 45.91% and 109.74% higher than those of dark treatment.Light has a regulatory effect on cell growth and exopolysaccharide synthesis,which provided theoretical guidance for efficient production of Ganodermalucidumexopolysaccharide.
作者
张业尼
钱磊
刘逸寒
孔庆学
张志军
ZHANG Yeni;QIAN Lei;LIU Yihan;KONG Qingxue;ZHANG Zhijun(College of Food Science and Bioteehnology,Tianjin AgriculturaI University,Tianjin 300384;Tianjin Research Institute of Forestry and Pomology,Tianjin 300384;Key Laboratory of Storage of Agro-produets,Ministry of Agriculture,Tianjin 300384;College of Bioteehnology,Tianjin University of Science and Technology,Tianjin 300457;Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin 300457)
出处
《北方园艺》
CAS
北大核心
2019年第2期154-159,共6页
Northern Horticulture
基金
天津市科技计划资助项目(17ZXBFNC00160)
天津市蔬菜现代农业产业技术体系创新团队资助项目(ITTVRS2017008)
天津市"131"创新型人才团队资助项目(20180337)
关键词
灵芝
光照
生物量
胞外多糖
蓝光
Ganoderma lucidum
illumination
biomass
exopolysaccharide
bluelight
