摘要
目的研究IL-22基因多态性(single nucleotide polymorphism,SNP)在乙型肝炎病毒相关性慢加急性肝衰竭(hepatitis B virus related acute-on-chronic liver failure,HBV-ACLF)的表达及临床预后判断价值。方法2016年1月至2017年6月就诊福建医科大学附属第一医院肝病中心的HBV-ACLF患者,采用imLDR^TM多重SNP分型试剂盒检测IL-22基因rs2227478、rs2227491、rs1179251、rs1179249、rs2227473、rs2227484、rs11611206共7个位点SNP分型,比较SNP基因型及等位基因分布与3个月死亡率的关系。组间基因型及等位基因型频数的比较采用R×C表的卡方检验或Fisher确切概率法,采用二元logistic回归分析IL-22基因多态性是否为ACLF患者独立预后因素。结果最终纳入研究的HBV-ACLF患者122例,其中男性92例(75.41%),女性30例(24.59%)。32例(26.23%)患者死亡(死亡组),90例(73.77%)患者存活(存活组)。IL-22基因rs2227484位点的基因型在死亡组与存活组间分布差异有统计学意义(χ2=6.128,P=0.033),死亡组A等位基因频率为15.6%,高于存活组的5.6%,差异有统计学意义(OR=0.318,95% CI:0.126~0.804,P=0.012);而其他6个位点(rs2227478、rs2227491、rs1179251、rs1179249、rs2227473、rs11611206)的基因型及等位基因在两组间分布差异均无统计学意义(均P>0.05)。二元logistic回归显示,IL-22基因rs2227484的多态性并非短期预后的独立危险因素(调整OR=3.102,95%CI:0.939~10.250,P=0.063)。结论IL-22基因rs2227484位点上AA基因型和A等位基因可能与HBV-ACLF患者的短期预后相关。
Objective To investigate the association between interleukin-22 (IL-22) single nucleotide polymorphisms (SNPs) and the prognosis of hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF). Methods The patients with HBV-ACLF from the First Affiliated Hospital of Fujian Medical University were retrospectively studied.Seven SNP genotypes of IL-22 gene, including rs2227478, rs2227491, rs1179251, rs1179249, rs2227473, rs2227484, and rs11611206, were detected using imLDR^TM multiple SNP typing kit and the distribution features of SNP genotypes were described.The relationship between the distribution of SNP genotypes and alleles and the prognosis of ACLF was analyzed.Comparison of genotypes and allele frequencies between groups were performed by chi-square test of R×C table or Fisher's exact tests.Binary logistic regression analysis was used to analyze whether IL-22 gene polymorphisms was an independent prognostic factor for patients with ACLF. Results A total of 122 patients with HBV-ACLF were included in this study.Ninety-two (75.1%) were male and 30 (24.59%) were female.Patients were stratified as survival group (90 cases) and non-survival group (32 cases) according to the Results of three months follow-up.The genotype distribution of rs2227484 of IL-22 gene was significantly different between the two groups (χ2=6.128, P=0.033).The A allele frequency in the non-survival group (15.6%) was significantly higher than that in the survival group (5.6%) with statistically significance (OR=0.318, 95% CI=0.126-0.804, P=0.012).There was no significant difference in the other six SNP genotypes of IL-22 gene between the two groups (all P >0.05).However, binary logistic regression showed that rs2227484 of IL-22 gene was not an independent risk factor for the short-term mortality in HBV-ACLF patients (adjusted OR=3.102, 95%CI: 0.939-10.250, P=0.063). Conclusions The A allele and AA genotype of rs2227484 of IL-22 gene may be associated with a short-term prognosis in patients with HBV-ACLF.
作者
林苏
周卿
林烨鸿
王明芳
朱月永
Lin Su;Zhou Qing;Lin Yehong;Wang Mingfang;Zhu Yueyong(Liver Research Center,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China)
出处
《中华传染病杂志》
CAS
CSCD
2018年第12期730-735,共6页
Chinese Journal of Infectious Diseases
基金
福建省医学创新项目(2016-cx-033)
福建自然科学基金项目(2017101187,2016Y0040).
