摘要
以‘冬枣’及其5个自然变异材料为试材,对其果实形态、果实营养、物候期、染色体倍性以及DNA指纹进行了比较分析,以期筛选出综合性状优异的‘冬枣’变异类型。结果表明:与普通‘冬枣’相比,变异1号材料为大果型,其单果质量显著增加,为25.22g,果实可溶性糖含量和糖酸比提高。在物候期上,变异1号和2号材料与‘冬枣’表现相近,但其它3个变异材料的果实成熟期比‘冬枣’晚约15d。流式细胞仪分析显示5个自然变异材料均为二倍体,15对SSR引物检测表明5个自然变异材料与‘冬枣’基因组DNA的SSR指纹相同,可能均为‘冬枣’的自然芽变。评价结果显示,变异1号属于优良大果变异,变异3、4、5号为晚熟变异。
Ziziphus jujuba Mill.'Dongzao'and its five natural variants were taken as materials,comparisons of fruit morphology,fruit nutrition,phonological period,chromosome ploidy and DNA fingerprint were carried out,in order to selecte the variation type of 'Dongzao'with excellent comprehensive charaters.The results showed that compared with 'Dongzao',the variant No.1 had significantly bigger fruits with the single fruit weight of 25.22 g and higher soluble sugar content and acid-sugar ratio.The phonological period of variation No.1 and No.2 was similar to that of'Dongzao',yet other three variants fruits matured 15 days later than 'Dongzao'.All the five natural variants were diploidy according to flow cytometry analysis.No difference of DNA fingerprint among'Dongzao'and five natural variants were observed using 15 pairs of SSR primers,which indicated that the five variants were the most probably bud mutations from 'Dongzao'.The evaluation demonstrated that the variant No.1was a big fruit type and the variant No.3,No.4 and No.5 belonged to late-maturing fruit type.
作者
罗智
郑兴娟
王利虎
伊魁林
王玖瑞
刘孟军
LUO Zhi;ZHENG Xingjuan;WANG Lihu;YI Kuilin;WANG Jiurui;LIU Mengjun(College of Forestry,Hebei Agricultural University,Baoding,Hebei 071001;College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071001;Research Ceriter of Chinese Jujube,Hebei Agricultural University, Baoding,Hebei 071001;Zhanhua Institute of Dongzao,Zhanhua,Shandong 256803;Beijing Collaborative Innovation Center for Eco-environmental Improvement With Forestry and Fruit Trees,Beijing 100000)
出处
《北方园艺》
CAS
北大核心
2019年第1期74-78,共5页
Northern Horticulture
基金
河北省科技计划资助项目(16226313D-1)
北京市2011协同创新中心资助项目(PXM2018_014207_000024)
天津市科技计划资助项目(17ZXBFNC00210)
关键词
'冬枣’
变异
果实
物候期
Ziziphus jujuba Mill
‘Dongzao'
variant
fruit
phenological period
