摘要
以甘草酸提取率为指标,优化了复合酶和超声辅助两步法提取甘草中甘草酸的最佳工艺条件。结果表明,甘草原料为1g时,复合酶法提取阶段的最佳工艺条件为:复合酶比例(果胶酶纤维素酶)1:3 (U/U),复合酶用量175U/g·底物,pH 5.5,时间1h,温度50℃,原料粒度40目,料液比1:25 (g/mL);超声辅助提取阶段的最佳工艺条件为:提取溶剂20%乙醇,料液比1:35 (g/mL),超声功率350W,时间15min,温度40℃。在最佳工艺条件下,甘草酸提取率可达(90.44±0.14)%。
Taking glycyrrhizic acid extraction rate was used as an index,the technological conditions of extraction of glycyrrhizic acid from Licorice wad optimized by two-stage method of combined enzymes and ultrasonic.When the mass of glycyrrhizic acid was 1 g,the optimum technological conditions for the enzymatic stage were as follows:the optimal ratio of combined enzymes(pectinase cellulose)was 1:3(U/U);the dosage of combined enzymes was175 U/g·Substrate,reacted at pH 5.5 for 1 hat 50℃;and the particle size of raw material was 40 mesh with the ratio of material to liquid 1:25(g/mL).The optimum technological conditions for the ultrasonic stage were as follows:the ultrasonic extraction solvent is20% ethanol,and the ratio of material to liquid is 1:35(g/mL),ultrasonic treating for 15 min at 40℃ with 350 W.Under these optimum conditions,the extraction rate of glycyrrhizic acid could reach(90.44±0.14)%.
作者
梁霞
吕晓玲
LIANG Xia;LU Xiao-ling(School of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)
出处
《食品与机械》
CSCD
北大核心
2018年第10期145-151,157,共8页
Food and Machinery
基金
国家重点研发计划资助项目(编号:2016YFD0400803)
关键词
复合酶
超声
提取
甘草酸
果胶酶
纤维素酶
combined enzymes
ultrasonic
extraction
glycyrrhizic acid
pectinase
cellulase
