摘要
目的探讨大黄素-8-O-β-D-吡喃葡萄糖苷对牙龈卟啉单胞菌(P. gingivalis)唾液酸酶活性及其毒力基因表达的影响。方法使用不同质量浓度的大黄素-8-O-β-D-吡喃葡萄糖苷(0.2、0.5、2、5、10 mg/mL)处理P. gingivalis W83(实验组),用未加药物的P. gingivalis W83作对照(对照组),采用荧光法检测大黄素-8-O-β-D-吡喃葡萄糖苷对P. gingivalis唾液酸酶活性的作用。5 mg/mL大黄素-8-O-β-D-吡喃葡萄糖苷作用于P. gingivalis W83,Real-time PCR法检测毒力基因fimA、fimR、fimS、kgp、rgpA和rgpB的表达情况。结果大黄素-8-O-β-D-吡喃葡萄糖苷对P. gingivalis唾液酸酶活性产生了抑制作用,当其质量浓度为0.2、0.5、2、5、10 mg/mL时,对唾液酸酶活性的抑制率分别为11.4%、32.23%、40.21%、73.54%、84.31%。与对照组比较,实验组(5 mg/mL大黄素-8-O-β-D-吡喃葡萄糖苷处理)的fimA、fimR、fimS、kgp、rgpA和rgpB基因表达均下降,差异均有统计学意义(均P <0.05)。结论大黄素-8-O-β-D-吡喃葡萄糖苷可有效抑制P. gingivalis唾液酸酶活性,其抑制作用会降低细菌毒力基因表达,有望成为预防及治疗牙周炎的新型药物。
Objective To detect the inhibitory effect of emodin-8-O-β-D-glucopyranoside on sialidase activity and virulence genes expression of Porphyromonas gingivalis(P. gingivalis). Methods Different concentrations of emodin-8-O-β-D-glucopyranoside(0.2,0.5,2,5,10 mg/mL)were added in P. gingivalis W83 cultural medium(experiment groups). The control group consisted of the untreated P. gingivalis W83. The inhibitory effect of emodin-8-O-β-D-glucopyranoside on P. gingivalis sialidase was verified by using fluorometric assay. At a concentration of emodin-8-O-β-D-glucopyranoside(5 mg/mL),the effect on virulence genes expression in P. gingivalis(fimA,fimR,fimS,kgp,rgpA and rgpB)was determined by Real-time PCR. Results When the concentrations were 0.2,0.5,2,5 and 10 mg/mL,the inhibition rates of sialidase activity were 11.4%、32.23%、40.21%、73.54% and 84.31%,respectively. Compared with control group,the expressions of fimA,fimR,fimS,kgp,rgpA and rgpB in experiment groups were decreased significantly(P < 0.05). Conclusion Emodin-8-O-β-D-glucopyranoside exhibits effective inhibition on sialidase activities in P.gingivalis,and decreases the expression of gene virulence,which may become a new drug in the prevention and treatment of periodontitis.
作者
喻施文
樊晓淼
陈晨
刘培成
李琛
YU Shi-wen;FAN Xiao-miao;CHEN Chen;LIU Pei-cheng;LIChen(Department of Periodontics,School and Hospital of Stomatology,China Medical University,Shenyang 110002,China)
出处
《中国实用口腔科杂志》
CAS
2019年第1期21-25,共5页
Chinese Journal of Practical Stomatology
基金
国家自然科学基金(81200785)
辽宁省自然科学基金(2015010496-301)
