摘要
为了筛选与人类内源性逆转录病毒囊膜蛋白syncytin具有相互作用的蛋白质,首先分别构建诱饵质粒Syncytin-pKT25与基因组文库质粒,然后共转入报告菌株DHM1感受态细胞中,并采用抗性筛选得到含有互作蛋白质的菌株。结果发现,筛选到一条包括31个氨基酸的短肽。进一步通过回复杂交和Co-IP验证,确定筛选到的蛋白质与syncytin是否具有相互作用。实验结果表明,筛选到一条与syncytin具有相互作用的氨基酸短肽SJ-1。
In order to screen proteins that interact with the envelope protein syncytin of human endogenous retroviruses, the bait plasmid Syncytin-pKT25 and genomic library plasmids were constructed respectively, and they were co-transfected into the competent reporter strain DHM1 cells. After screening for antibiotic resistance, the strains containing the interacting proteins were identified and a short peptide consisting of 31 amino acids was found. The interaction between syncytin and the peptide was verified by recombination and Co-IP validation. The result demonstrated preliminarily that the short peptide named SJ-1 is an interacting peptide with syncytin.
作者
井敏敏
司徒健文
陶瑞
范欣
井申荣
孙鹥
JING Min-min;SITU Jian-wen;TAO Rui;FAN Xin;JING Shen-rong;SUN Yi(Medical Faculty,Kunming University of Scienoe and Technology,Kunming 650500,Yunnan,China;Department of Clinical Laboratory,the First People's Hospital of Yunnan Province,Kunming 650032,Yunnan,China)
出处
《生命科学研究》
CAS
CSCD
2018年第6期438-444,共7页
Life Science Research
基金
国家自然科学基金资助项目(81100377)
云南省应用基础研究项目(2013FB203
31060155)
