摘要
目的通过检测甲状腺功能减退(简称甲减)对雄性大鼠睾丸组织细胞外信号调节激酶(ERK1和ERK2)基因mRNA表达的影响,探讨甲减造成雄性生殖系统损伤的可能机制。方法健康雄性Wistar大鼠30只,采用随机数字表法,按体重随机分为3组:对照组(C组,灌胃生理盐水1ml/100g·bw),低剂量组[L组,灌胃0.1mg/kg·bw丙基硫氧嘧啶(PTU)1ml/100g·bw]和高剂量组(H组,灌胃10.0mg/kg·bw PTU 1ml/100g·bw),每组10只,灌胃60d。灌胃结束次日处死大鼠,采用放射免疫法检测血清甲状腺激素水平[总三碘甲状腺素原氨酸(TT3)、总甲状腺素(TT4)和促甲状腺激素(TSH)];实时荧光定量PCR(RT-qPCR)检测睾丸组织ERK1和ERK2基因mRNA表达水平。结果灌胃60d,与C和L组TT3[(0.86±0.07)和(0.79±0.06)nmol/L]和TT4[(45.56±1.52)和(39.02±1.33)nmol/L]相比,H组TT3[(0.39±0.01)nmol/L]和TT4[(15.47±1.21)nmol/L]均明显下降,差异有统计学意义(P<0.05)。与C组和L组TSH[(0.18±0.06)和(0.27±0.05)mU/L]相比,H组TSH[(0.65±0.09)mU/L]水平明显上升,差异有统计学意义(P<0.05)。与C组ERK1及ERK2基因表达水平(1.2433±0.4694,1.1138±0.3666)相比,L组ERK1(0.9617±0.5014)及ERK2基因(0.7007±0.4644)表达水平虽有所下降,但无统计学意义(P >0.05),H组ERK1(0.4848±0.2251)及ERK2(0.4028±0.1882)基因表达水平明显降低(P<0.05)。结论甲减可能通过抑制睾丸组织ERK1和ERK2表达水平影响大鼠生殖系统。
Objective To define the effect of hypothyroidism on extracellular signal regulated kinase 1 and 2(ERK1 and ERK2) mRNA expression in testes of male rats, in order to explore the mechanism of hypothyroidism on male reproduction. Methods According to body weight(240~270 g), thirty male Wistar rats were divided into control group(C group, 1ml/kg·bwd normal saline),low-dose group(L group, 0.1 mg/kg·bw PTU1 ml/kg·bw·d) and high-dose group(H group, 10 mg/kg·bw PTU 1 ml/kg·bw·d) by intragastric administration and body weights were weighed once every 3 days. After 60 days, all rats were executed. The levels of thyroid hormones, including total triiodothyronine(TT3), total thyroxine(TT4), and thyroid stimulating hormone(TSH), were measured by radioimmunoassay. The mRNA expression levels of ERK1 and ERK2 in testes were detected by real-time quantitative PCR(RT-qPCR).Result Compared with the serum levels of TT3 [(0.86±0.07)nmol/L,(0.79±0.06)nmol/L] and TT4 [(45.56±1.52)nmol/L,(39.02±1.33)nmol/L] in C and L group, TT3 [(0.39±0.01)nmol/L] and TT4[(15.47±1.21)nmol/L]in H group were found to be significantly decreased(P<0.05). Whereas, the level of TSH [(0.65±0.09)mU/L)]was significantly increased compared with the C group [(0.18±0.06)mU/L] and L group[(0.27±0.05) mU/L, P<0.05]. Moreover, compared with the mRNA expression of ERK1(1.2433±0.4694) and ERK2(1.1138±0.3666) in C groups, ERK1(0.9617±0.5014) and ERK2(0.7007±0.4644) mRNA expression in L group were no significantly decreased(P >0.05), while, there were significant decrease of ERK1(0.4848±0.2251) and ERK2(0.4028±0.1882) in H group(P<0.05).Conclusion Hypothyroidism may inhibit the ERK1 and ERK2 mRNA expression of MAPK cell signaling pathway and then damage the reproductive system in male rats.
作者
姚月丽
王惠玲
马海涛
王栋
常晓茹
冯君平
王俊玲
YAO Yue-li;WANG Hui-ling;MA Hai-tao;WANG Dong;CHANG Xiao-ru;FENG Jun-ping;WANG Jun-ling(Department of Toxicology,School of Public Health,Lanzhou University,Lanzhou Gansu 730000,China;Department of Integrated Chinese and Western Medicine Gynecology,Gansu Provincial Maternity and Child-care Hospital,Lanzhou Gansu 730000,China;Key Laboratory of Environmental Medicine and Engineering,Ministry of Education,School of Public Health,Southeast University,Nanjing Jiangsu 210000,China;Lanzhou Biological products Research Institute Co.,Ltd/Gansu Provincial Vaccine Engineering Technology Research Center,Lanzhou Gansu 730000,China)
出处
《毒理学杂志》
CAS
CSCD
2018年第6期443-447,共5页
Journal of Toxicology
基金
国家自然科学基金(81550046)
兰州大学中央高校基本科研业务费专项资金(lzujbky-2017-it34)
