摘要
利用混合自组装的方式,将Mb功能化纳米金(Mb-AuNPs-MUA)修饰在金电极表面,以制备出检测超氧阴离子无电子媒介体生物传感器.采用UV-Vis考察修饰纳米团簇的相关特征,利用修饰电极检测DMSO/NaOH体系产生的超氧阴离子.试验结果表明:该修饰电极对超氧阴离子的歧化反应具有显著的催化活性,计算出异相电子传递速率常数(Ks)为0.041 cm/s,电子转移系数(α)为0.435.在0.06～0.2μmol/L范围内,超氧阴离子浓度与峰电流呈良好的线性关系,相关系数R^2为0.9719,方法检出限(LOD)为1.129×10^(-3)μmol/L(S/N=3)、3.683×10^(-3)μmol/L(S/N=10),精密度试验测定得相对标准偏差(RSD,n=9)为3.83%.
Mb-functionalized gold nanoparticles (Mb-AuNPs-MPA) was modified on the gold electrode surface by a mixed self-assembly method to prepare a biosensor that detects superoxide anion mediators of free electron. UV-Vis were used to investigate the characteristics of the modified nanoclusters. Superoxide anions were determined by the modified electrodes in the basic DMSO system. The experimental results demonstrated that the modified electrodes had remarkable ability to catalyze the dismutation of the superoxide anion. The electron transfer coefficient (α) and the heterogeneous rate constant(Ks) were calculated to be 0.435 and 0.041 cm/s, respectively. The linear relationship between the superoxide anions content and peak current was better in the range of 0.06~0.2 μmol/L(R^2=0.9719). The detection limit were 1.129×10^-3 μmol/L(S/N=3) and 3.683×10^-3 μmol/L(S/N=10). Relative standard deviation(RSD) determined by precision test(n=9) was 4.26%.
作者
陈林林
王振兴
窦博鑫
韩可
辛嘉英
CHEN Lin-lin;WANG Zhen-xing;DOU Bo-xin;HAN Ke;XIN Jia-ying(Key Laboratory for Food Science and Engineering,Harbin University of Commerce,Harbin 150076,China;State Key Laboratory for Oxo Synthesis and Selective Oxidation,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China)
出处
《分子催化》
EI
CAS
CSCD
北大核心
2018年第6期574-580,I0004,共8页
Journal of Molecular Catalysis(China)
基金
国家自然科学基金项目(21573055)
哈尔滨市应用技术研究与开发项目(2014RFQXJ115)
哈尔滨商业大学校级科研项目(18XN079)~~
关键词
甲烷氧化菌素
纳米金
电化学
超氧阴离子
methanobactin
gold nanoparticles
electrochemical
superoxide anion
