摘要
目的应用超声心动图评价经野百合碱(MCT)诱导的肺动脉高压大鼠右室功能,并探讨17β-雌二醇(E2)对肺动脉高压大鼠右室功能的作用。方法选取8周龄雄性SD大鼠32只,随机平均分为4组(每组8只):MCT组、MCT+E2组,MCT+安慰剂(Veh)组和对照组。MCT组、MCT+E2组及MCT+Veh组大鼠均予以1%野百合碱腹腔注射。MCT+E2组及MCT+Veh组大鼠分别予以E2或安慰剂经皮下微量泵持续泵入。MCT注射4周后对所有大鼠进行超声心动图检查。超声心动图检查完毕后进行右心导管测压。超声心动图右室功能测量参数包括三尖瓣环收缩期位移(TAPSE)、右室心肌做功指数(RIMP)、右室面积变化分数(RVFAC)、三尖瓣环收缩期峰值速度(s')及右室游离壁纵向应变(RVLSFW)。结果与对照组相比,MCT组TAPSE、RVFAC、s'、RVLSFW降低,RIMP升高,差异有统计学意义(分别P<0.001,P<0.001,P<0.001,P=0.001,P<0.001);MCT+E2组TAPSE、RVFAC、RVLSFW高于MCT组,RIMP低于MCT组(分别P<0.001,P=0.001,P=0.001,P=0.002,P=0.002);MCT+E2组右室功能参数虽较对照组有所减低,但差异无统计学意义(P>0.05)。Pearson相关性分析结果示大鼠E2浓度与TAPSE、RVFAC、s′及RVLSFW呈正相关(分别r=0.845,P<0.001;r=0.859,P<0.001;r=0.802,P=0.006),与RIMP呈负相关(r=-0.803,P<0.001)。结论经MCT诱导的肺动脉高压大鼠右室功能减低,E2可以改善肺动脉高压大鼠右室功能不全,对肺动脉高压右室功能具有保护作用。超声心动图在定量评价肺动脉高压大鼠右室功能中具有重要作用。
Objective To evaluate right ventricular(RV)function in male rats with pulmonary arterial hypertension(PAH)induced by Monocrotaline(MCT)and discuss the effect of 17β-estradiol(E2)on RV function in PAH rats.Methods Thirty-two male SD rats were randomly divided into 4 groups(n=8):MCT group,MCT+E2 group,MCT+Veh group and control group.MCT group,MCT+E2 group and MCT+ Veh group were given monocrotaline through intraperitoneal injection.MCT+E2 group and MCT+Veh group were also treated with E2 and placebo at the same time respectively.Echocardiography was performed four weeks later after MCT injection.Right heart catheterization was performed after echocardiography.Echocardiography right ventricular function parameters included:tricuspid annular plane systolic excursion(TAPSE),RV index of myocardial performance(RIMP),right ventricular fractional area change(RVFAC),tissue Doppler-derived tricuspid lateral annular systolic velocity(s')and right ventricular free wall longitudinal strain(RVLSFW).Results Compared with control group,TAPSE,RVFAC and RVLSFW decreased,and RIMP increased in MCT group,(P<0.001,P<0.001,P<0.001,P=0.001,P<0.001,respectively).TAPSE,RVFAC and RVLSFW in MCT E2 group were higher and RIMP was lower than those in MCT group(P<0.001,P=0.001,P=0.001,P=0.002,P=0.002,respectively).The parameters regarding RV function were slightly lower in MCT+E2 group,compared with control group.However,these differences had no obvious statistical significance(P>0.05).Moreover,Pearson correlation analysis showed that the serum E2 level was positively correlated with TAPSE,RVFAC,s'and RVLSFW(r=0.845,P<0.001;r=0.859,P<0.001;r=0.802,P=0.006,respectively),and negatively correlated with RIPM(r=-0.803,P<0.001).Conclusions RV function in male PAH rats induced by MCT decreased significantly,while E2 could improve RV function in PAH rats and has a protective effect on RV function.Echocardiography could contribute to the quantitative evaluation of RV function in PAH rats induced by MCT.
作者
王怡丹
李一丹
丁雪晏
李成
吴小朋
郭迪晨
石艳萍
吕秀章
Wang Yidan;Li Yidan;Ding Xueyan;Li Cheng;Wu Xiaopeng;Guo Dichen;Shi Yanping;Lyu Xiuzhang(Department of Echocardiography,Beijing Chao Yang Hospital, Capital Medical University,Beijing 100020,China)
出处
《中华超声影像学杂志》
CSCD
北大核心
2018年第12期1089-1094,共6页
Chinese Journal of Ultrasonography
基金
国家自然科学基金项目(81401420).
关键词
超声心动描记术
高血压
肺性
心室功能
右
雌二醇
大鼠
Echocardiography
Hypertension,pulmonary
Ventricular function,right
Estradiol
Rat
