摘要
目的观察温阳败毒饮对脓毒症大鼠血清肝脏DeRitis比值〔即天冬氨酸转氨酶/丙氨酸转氨酶(AST/ALT)比值〕和肾脏中性粒细胞明胶酶相关脂质运载蛋白(NGAL)水平的影响。方法选择120只SD大鼠,按随机数字表法将大鼠分为正常对照组、模型组、西药治疗组、中药治疗组、联合用药组,每组24只。采用腹腔内注射大肠杆菌内毒素的方法制备大鼠脓毒症模型;制模后2h西药治疗组给予地塞米松2.5mg/kg灌胃;中药治疗组给予温阳败毒饮(金银花30g、蒲公英30g、附子30g,水煎2次,所得药液浓缩制成干膏备用,使用时用温水溶化,每日2次)8g/kg灌胃;联合用药组给予温阳败毒饮+地塞米松灌胃;正常对照组、模型组给予生理盐水灌胃。比较制模后24、48、72h各组DeRitis比值及NGAL水平的差异。结果模型组制模后24、48、72hDeRitis比值和NGAL水平均较正常对照组明显升高〔DeRitis比值分别为4.14±0.53比2.08±0.37、4.78±0.07比1.73±0.61、4.83±0.31比1.05±0.20,NGAL(ng/L)分别为2412.73±368.03比837.52±142.98、1735.16±290.76比817.38±102.08、1838.43±295.44比847.82±140.14,均P<0.05〕。西药治疗组、中药治疗组和联合用药组各时间点DeRitis比值和NGAL均较模型组明显降低,且以联合用药组的降低程度更显著,制模后48h联合用药组与西药治疗组、中药治疗组比较出现统计学差异〔DeRitis比值:3.09±0.15比3.68±0.44、3.27±0.62,NGAL(ng/L):1105.32±270.27比1206.35±143.82、1526.53±289.02〕,持续到72h达到最低水平〔DeRitis比值:2.27±0.26比2.93±0.38、2.61±0.54,NGAL(ng/L):903.47±105.37比9316.05±116.23、1025.14±152.68,均P<0.05〕。联合用药组在改善脓毒症大鼠DeRitis比值和NGAL方面较单用西药或单用中药具有明显优势。结论温阳败毒饮具有有效降低内毒素诱导的脓毒症大鼠肝脏DeRitis比值、肾脏NGAL水平的作用。
Objective To observe the effects of Wenyang Baidu Yin on the serum liver DeRitis ratio [aspartate aminotransferase/alanine aminotransferase (AST/ALT) ratio] and the level of renal neutrophil gelatinaseassociated lipocalin (NGAL) in septic rats. Methods One hundred and twenty healthy male sprague-dawley (SD) rats were divided into normal control group, model group, western medicine treatment group, traditional Chinese medicine (TCM) treatment group and combined medicine group by random number table method, with 24 rats in each group. Rat sepsis model was established by intra-peritoneal injection of E.coli endotoxin; after modeling for 2 hours, normal control group and model group were given normal saline intragastric administration, western medicine treatment group was given dexamethasone 2.5 mg/kg intra-gastric administration, TCM treatment group was given Wenyang Baidu Yin intragastric administration (honeysuckle 30 g, dandelion 30 g, aconite 30 g, mixed with water and after two times of boiling, the obtained liquid was concentrated and made into dry ointment for reserve, when the ointment was used, it was dissolved in warm water to 8 g/kg, administered by gavage, twice a day), the combined medicine group was given both the above TCM decoction and dexamethasone dosages by gavage. After modeling for 24, 48 and 72 hours, the differences of DeRitis ratio and NGAL levels were compared among various groups. Results The DeRitis ratio and NGAL level in the model group were significantly higher than those in the normal control group at 24, 48, 72 hours after modeling (DeRitis ratio: 4.14±0.53 vs. 2.08±0.37, 4.78±0.07 vs. 1.73±0.61, 4.83±0.31 vs. 1.05±0.20 respectively, NGAL was 2 412.73±368.03 vs. 837.52±142.98, 1 735.16±290.76 vs. 817.38±102.08, 1 838.43±295.44 vs. 847.82±140.14 respectively, all P < 0.05). The DeRitis ratios and NGAL levels of western medicine treatment group, TCM treatment group and combined medicine group were significantly lower than those of model group at each time point, and the decr
作者
张铄
梁静
王素娟
Zhang Shuo;Liang Jing;Wang Sujuan(Department of Intensive Care Unit, Shanxi Provincial Hospital of Integrated Traditional Chinese and Western Medicine,Taiyuan 030013, Shanxi, China)
出处
《中国中西医结合急救杂志》
CAS
CSCD
北大核心
2018年第6期636-638,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
山西省医药卫生科研课题(2014106).
