摘要
目的研究柯萨奇病毒A16型(CV-A16)疫苗候选株不同形态病毒颗粒与不同佐剂配伍后在小鼠模型中的免疫原性和保护性.方法将不同佐剂与不同剂量的CV-A16疫苗候选株纯化抗原配制成疫苗,免疫小鼠后检测小鼠血清内抗CV-A16的总抗体水平以及中和抗体水平,并评价母传抗体对新生乳鼠的保护作用.结果CV-A16疫苗候选株可在小鼠体内诱导高水平的体液免疫应答,免疫后的小鼠血清中和抗体水平可达8192,并可交叉中和CV-A16病毒原型株(A型)以及异源亚基因型(B2b).疫苗免疫母鼠生产的乳鼠在被10000LD50的同源或异源CV-A16病毒攻击后,可免于发病和死亡.结论CV-A16疫苗候选株具有良好的免疫原性和保护性,可作为CV-A16灭活疫苗或多价手足口病灭活疫苗的候选毒株.
Objective To evaluate the immunogenicity and protective effect of CV-A16 vaccine candidate strains in a mouse model. Methods Different doses of the full virus particles or empty virus particles of CV-A16 vaccine candidate strains(inactivated or not inactivated by formalin)were formulated with different adjuvants into vaccine preparations.After immunization,the anti-CV-A16 IgG antibody and neutralizing antibody levels in the serum of female mice were detected.The pups from immunized female mice were challenged with homologous and heterologous viruses. Results It was showed that the L731 vaccine induced robust immune responses in mice.Mouse antisera contained the highest titers of neutralizing antibodies reported to date and prevented cells from infections by prototype A and subtype B2 bof CV-A16 in vitro.Pups born to immunized mice were protected from disease and death after being challenged with 10 000 LD50 of homologous and heterologous viruses. Conclusions L731 is a promising vaccine candidate that can be used alone or as a component of multivalent or combined vaccines.
作者
卢佳
张林林
安泰学
毛群颖
李鹏飞
张夫坤
王泽鋆
李黎
周辉
王继麟
陈晓琦
梁争论
申硕
LU Jia;ZHANG Lin-lin;AN Tai-xue;MAO Qun-ying;LI Peng-fei;ZHANG Fu-kun;WANG Ze-jun;LI Li;ZHOU Hui;WANG Ji-lin;CHEN Xiao-qi;LIANG Zheng-lun;SHEN Shuo(Laboratory of Viral Vaccine Research,Wuhan Institute of Biological Product ( WIBP ) Ltd.Co.,Wuhan,Hubei 430207,China)
出处
《中国病毒病杂志》
CAS
2018年第6期487-494,共8页
Chinese Journal of Viral Diseases
基金
国家“重大新药创制”科技重大专项课题(2015ZX09102021)
2017年湖北省技术创新专项(重大项目)基金支持(2017ACA078)
