摘要
目的探究可溶性血管内皮细胞生长因子受体1(sFlt-1)与溶酶体相关膜蛋白2(LAMP-2)在抗中性粒细胞胞质抗体(ANCA)相关性肾炎患者血清中的表达水平及其相关性。方法选择2016年10月至2017年10月间90例ANCA相关性肾炎患者作为研究组,根据24h尿蛋白水平分为低蛋白尿组和高蛋白尿组。选择同期单纯慢性肾小球肾炎患者共90例作为对照组以及50例健康者作为健康组。分别比较不同组间的血清和尿液中的sFlt-1和LAMP-2水平,并分别研究sFlt-1和LAMP-2水平与ANCA相关性肾炎以及24h尿蛋白的相关性。结果研究组血清和尿液中的sFlt-1和LAMP-2水平均显著高于对照组和健康组(P<0.05)。将尿液和血清中的sFlt-1和LAMP-2纳入Logistic多元回归分析,结果显示血清LAMP-2以及尿液LAMP-2是ANCA相关性肾炎的高危因素(P<0.05)。在ANCA相关性肾炎患者(研究组)中,高蛋白尿组患者血清和尿液中的sFlt-1和LAMP-2水平均显著高于低蛋白尿组(P<0.05),并且均显著高于健康组(P<0.05)。血清和尿液中sFlt-1和LAMP-2与ANCA相关性肾炎患者24h尿蛋白呈现显著的正相关性(P<0.05)。结论慢性肾小球肾炎患者血清和尿液中sFlt-1和LAMP-2水平显著升高,ANCA相关性肾炎患者LAMP-2水平显著高于慢性肾小球肾炎患者,并且血清和尿液中LAMP-2是ANCA相关性肾炎的高危因素。
Objective To investigate the expression level and correlation of sFlt-1 and LAMP-2 in the serum of patients with ANCA-associated nephritis.Methods Ninety cases of ANCA-associated nephritis were selected as the study group from October 2016 to October 2017,and were classified into low-proteinuria subgroup and high-proteinuria subgroup according to 24-h proteinuria level.A total of 90 patients with chronic glomerulonephritis were selected as the control group and 50 healthy subjects as the healthy group.The levels of sFlt-1 and LAMP-2 in serum and urine samples from different groups were compared,and the correlation between sFlt-1 and LAMP-2 levels with ANCA-associated nephritis and 24-h urine protein was analyzed.Results The levels of sFlt-1 and LAMP-2 in the serum and urine samples of the study group were significantly higher than those in the control group and the healthy group(P<0.05).Logistic multiple regression analysis of sFlt-1 and LAMP-2 in urine and serum showed that serum LAMP-2 and urine sample LAMP-2 were risk factors for ANCA-associated nephritis(P<0.05).In patients with ANCA-associated nephritis(study group),the levels of sFlt-1 and LAMP-2 in serum and urine samples of patients with high proteinuria were significantly higher than those of low proteinuria(P<0.05),and healthy group(P<0.05).There was a significant positive correlation between sFlt-1 and LAMP-2 in serum and urine samples with 24-h urine protein in patients with ANCA-associated nephritis(P<0.05).Conclusions The levels of sFlt-1 and LAMP-2 in serum and urine of patients with chronic glomerulonephritis were significantly increased.The level of LAMP-2 in patients with ANCA-associated nephritis was significantly higher than that in patients with chronic glomerulonephritis,and LAMP-2 in serum and urine is a high risk factor for ANCA-associated nephritis.
作者
翟英
赵明
李向东
李毅
沈海燕
ZHAI Ying;ZHAO Ming;LI Xiang-dong;LI Yi;SHEN Hai-yan(Department of Nephrology, the 3201st Hospital of Hanzhong, Hanzhong 723000, China)
出处
《临床肾脏病杂志》
2018年第12期748-751,共4页
Journal Of Clinical Nephrology
