摘要
以富含蛋白的豆奶为碳源,通过一步水热法合成高荧光量子产率的氮掺杂荧光碳量子点(N-CQDs),透射电镜(TEM)观察N-CQDs的粒径在3nm左右; X射线光电子能谱(XPS)和傅里叶红外光谱(FTIR)证明N-CQDs的表面被羧基、氨基、羟基、羰基等官能团功能化,具有良好水溶性.在PBS缓冲液中(p H6. 0)中,N-CQDs的荧光强度(F)与环丙沙星浓度在0. 1～10. 0μg/m L之间存在良好的线性关系,检出限为0. 08μg/m L.研究发现,环丙沙星(CIP)对NCQDs有良好的荧光增敏作用,可作为荧光探针测定环丙沙星含量.建立的方法用于鸡蛋中环丙沙星的检测,具有方法灵敏度高、选择性好、简便快速等特点.
Nitrogen-doped fluorescent carbon quantum dots(N-CQDs) of high-fluorescence quantum were created by one-step hydrothermal synthesis,protein-rich soy milk being used as a carbon source. The N-CQDs particle size is observed about 3 nm by Transmission electron microscopy(TEM). The surface of N-CQDs has been proved to be functionalized with carboxyl,amino,hydroxyl,carbonyl and other functional groups by X-ray photoelectron spectroscopy(XPS) and Fourier transform infrared spectroscopy(FTIR). The water solubility was good. The fluorescence intensity(F) of N-CQDs in PBS buffer(pH6. 0) was linear with the concentration of ciprofloxacin in 0. 1-10. 0μg/m L. The limit of detection was 0. 08 μg/m L. Through research,Ciprofloxacin(CIP) has a good fluorescence sensitization to N-CQDs,which can be used as a fluorescent probe to determine the content of ciprofloxacin. The established method is applied to the detection of ciprofloxacin in eggs,which has the advantages of high sensitivity,good selectivity,simple and quick method.
作者
胡枫
杨亚玲
HU Feng;YANG Yaling(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)
出处
《昆明理工大学学报（自然科学版）》
CAS
北大核心
2018年第6期90-95,共6页
Journal of Kunming University of Science and Technology(Natural Science)
基金
国家自然科学基金项目(31460430
31660437)
关键词
碳量子点
荧光探针
增敏
环丙沙星
鸡蛋
Carbon quantum dots
Fluorescent probe
sensitizing
Ciprofloxacin
egg
