摘要
目的探讨一种成瘤周期短、操作便捷、安全性高、适用于肝癌治疗研究的SD大鼠原位种植型肝癌模型制作方法,同时进行彩色超声、增强CT、MR等3种影像学检查和诊断。方法 20只SD大鼠随机分为Mc A-RH7777组和N1S1组,每组10只。称重后将Mc A-RH7777和N1S1肝癌细胞分别注入两组大鼠肝左中叶内,术后连续3 d腹腔注射青霉素(20万U/只)和地塞米松(2.5 mg/只),以抗感染和免疫抑制治疗。术后7 d,采用彩色超声、增强CT、MR鉴别肝脏肿瘤,记录模型鼠体重,取出肿瘤组织进行形态学观察和病理学检查。结果术后7 d,Mc A-RH7777组、N1S1组大鼠分别死亡2只、1只,影像学检查显示成活大鼠全部成瘤,建模成功率分别为80%(8/10)和90%(9/10),差异无统计学意义(P=0.86);大鼠体重分别减轻(50.00±24.93) g、(36.44±7.75) g;肿瘤体积分别为(316.43±265.84) mm3、(188.58±137.47) mm3。两组大鼠处死后,尸检结果与影像学检查一致;肉眼观察Mc A-RH7777肝癌外观呈团块状,N1S1肝癌呈结节状。HE染色显示Mc A-RH7777肝癌为高分化肝癌,N1S1肝癌为低分化肝癌。结论该方法能成功、高效地构建SD大鼠原位肝癌模型,同时通过不同影像学技术实现诊断和评价,为肿瘤介入治疗研究提供了一种可供选择的实验工具。
Objective To explore the establishment method of in situ hepatocellular carcinoma(HCC) models in SD rats that have the advantages of short tumorigenic cycle, simple manipulation and high safety, for which three kinds of imaging examination and diagnosis(Doppler ultrasound, enhanced CT and MRI) can be employed. Methods A total of 20 SD rats were randomly divided into McA-RH7777 group and N1 S1 group, with 10 rats in each group. After weighing, Mc A-RH7777 HCC cells and N1 S1 HCC cells were injected into the left-middle hepatic lobe of the rats of McA-RH7777 group and N1 S1 group respectively.After the injection of HCC cells, intraperitoneal injection of penicillin(200 000 units/day/per rat) and dexamethasone(2.5 mg/day/per rat) was given for 3 days to make anti-infection and immunosuppression therapy. Seven days after the treatment, Doppler ultrasound, enhanced CT and magnetic resonance imaging(MRI) examinations were performed to identify the formation of liver tumor; the weight of each rat model was recorded, and the tumor tissues were taken out for morphological observation and pathological examination.Results Seven days after the treatment, 2 rats in McA-RH7777 group and one rat in N1 S1 group died.Imaging examinations indicated that successful tumor formation was achieved in all the surviving rats, the success rates of modeling in McA-RH7777 group and in N1 S1 group were 80%(8/10) and 90%(9/10)respectively, the difference between the two groups was not statistically significant(P=0.86). In McA-RH7777 group and in N1 S1 group, the body weight of SD rats were reduced by(50.00±24.93) g and by(36.44±7.75) g respectively, the tumor volumes were(316.43±265.84) mm3 and(188.58±137.47) mm3 respectively. After the rats were sacrificed, the autopsy results were consistent with imaging findings. Macroscopic observation showed that the appearance of McA-RH7777 HCC lesion took the shape of a lump, while N1 S1 HCC lesion was nodular in shape. Microscopic examination with HE staining revealed that McA-RH7777 HCC was highl
作者
李鑫
刘凤永
袁宏军
管阳
付金鑫
王茂强
LI Xin;LIU Fengyong;YUAN Hongjun;GUAN Yang;FU Jinxin;WANG Maoqiang(Department of Interventional Radiology, General Hospital of PLA, Beijing 100853, China)
出处
《介入放射学杂志》
CSCD
北大核心
2018年第12期1177-1181,共5页
Journal of Interventional Radiology
基金
国家自然科学基金面上项目(81671800)
北京市自然科学基金面上项目(7172204)
关键词
肝癌
大鼠
McA-RH7777肝癌细胞
N1S1肝癌细胞
动物模型
彩色超声
增强CT
磁共振成像
hepatocellular carcinoma
SD rat
McA-RH7777 hepatocellular carcinoma cell
N1S1hepatocellular carcinoma cell
animal model
Doppler ultrasound
enhanced CT scan
magnetic resonanceimaging
