摘要
目的:探讨血清HCV-RNA载量及抗-HCV浓度检测在丙型肝炎临床诊断中的应用。方法:选取2017-04—2018-04治疗的丙型肝炎患者100例纳入试验组,选取同期30例健康人群纳入对照组。采用荧光定量PCR法对其进行HCV-RNA载量检测,ELISA法检测抗-HCV浓度、全自动生化分析仪检测血清中AST、ALT、TBIL的浓度,并进行统计学分析。结果:100例标本中抗-HCV阳性者89例,阳性率为89%;随着HCV-RNA载量的不断增加,抗-HCV阳性率不断升高,差异有统计学意义(P<0.05);试验组的抗-HCV s/co值高于对照组,差异有统计学意义(P<0.05);试验组的血清AST、ALT、TBIL值均明显高于对照组,差异有统计学意义(P<0.05)。结论:HCV-RNA载量联合抗-HCV及肝功能指标检测,不仅可有效对患者的感染、病情严重程度做出准确判断,还可以对临床治疗进行监测。
Objective:To investigate the application of serum HCV-RNA load and anti-HCV concentration detection in the clinical diagnosis of hepatitis C.Method:A total of 100 patients with hepatitis C treated with 2017-04-2018-04 were enrolled in the experimental group.Thirty healthy people in the same period were selected as the control group.The HCV-RNA load was detected by real-time PCR,the anti-HCV concentration was detected by ELISA,and the concentrations of AST,ALT and TBIL in serum were detected by automatic biochemical analyzer,and statistical analysis was performed.Result:Of the 100 specimens,89 were positive for anti-HCV,and the positive rate was 89%.With the increase of HCV-RNA load,the positive rate of anti-HCV increased continuously,the difference was statistically significant(P<0.05).The anti-HCV s/co value of the experimental group was higher than that of the control group,and the difference was statistically significant(P<0.05).The serum AST,ALT and TBIL values of the experimental group were significantly higher than the control group,and the difference was statistically significant(P<0.05).Conclusion:HCV-RNA load combined with anti-HCV and liver function indicators can not only effectively determine the patient’s infection and severity,but also monitor clinical treatment.
作者
白娜
BAI Na(Department of Ciinical Laboratory,Pingdingshan First People's Hospital,Pingdingshan,467000,China)
出处
《临床血液学杂志(输血与检验)》
CAS
2018年第6期922-924,共3页
Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)