摘要
目的探讨结肠癌患者细胞核高尔基体受体复合物1(Golgi SNAP receptor complex 1,GS28)蛋白的表达情况以及对术后预后的影响。方法选取350例结肠癌患者作为研究对象,免疫组织化学法分析细胞核中GS28蛋白的表达情况。根据是否主要表达在细胞核,分为核优势表达组(n=48)和非核优势表达组(n=302)。比较两组患者的临床病理特征,分析细胞核GS28蛋白表达水平与鼠类肉瘤病毒癌基因(kirsten rat sarcoma viral oncogene,KRAS)突变及表皮生长因子受体(epidermal growth factor receptor,EGFR)表达水平相关性,并采用Kaplan-Meier法分析两组患者的总体生存期。结果本组350例中,GS28蛋白在细胞核中优势表达48例(13. 7%)。GS28核优势表达组和非核优势表达组患者在T分期和神经浸润方面比较,差异均具有统计学意义(均P <0. 05)。相关性分析显示,细胞核GS28蛋白表达水平与KRAS突变及EGFR表达水平均无相关性(P=0. 924,P=0. 126)。GS28核优势表达组患者中位生存期低于非核优势表达组(62个月vs 77个月,P <0. 05)。结论 GS28蛋白细胞核优势表达影响结肠癌患者的总体生存期,可以考虑作为潜在的预后标志物。
Objective To investigate the expression of nuclear Golgi SNAP receptor complex 1 (GS28) protein and its impacts on the postoperative survival of colon cancer patients. Methods Three-hundred-and-fifty colon cancer patients were recruited. The level of nuclear GS28 protein expression were examined by pathological and immunohistochemical analysis. According to whether mainly expressed in the nucleus,the patients were divided into nuclear predominant group(n = 48) and non nuclear predominant group (n =302). The clinical data and the relation between GS28 protein expression in the nuclear and mutation of kirsten rat sarcoma viral oncogene (KRAS) and epidermal growth factor receptor (EGFR) expression were analyzed. The overall survival was analyzed with the Kaplan-Meier method. Results Among the 350 cases, 48 cases has nuclear predominant GS28 expression (13. 7%);and there were statistically significant differences in the data about neural invasion and T stage between the nuclear predominant group and non-nuclear predominant group (both P < 0. 05). Correlation analysis showed that there was no correlation between GS28 protein expression in the nuclear and KRAS mutation or EGFR expression level(P =0. 924,P = 0. 126). The median survival time of nuclear predominant group was significantly lower than that of non-nuclear predominant group (62 months vs 77 months,P < 0. 05). Conclusion The nuclear dominance expression of GS28 protein affects the overall survival of colon cancer patients,and can be used as a potential prognostic marker.
作者
黄江
刘敏
陈绪阳
魏晴
Huang Jiang;Liu Min;Chen Xuyang;Wei Qing(Department of General Surgery,Affiliated Hospital of Yan'an University,Yan'an 716000,China;Department of Oncology,Affiliated Hospital of Yan'an University,Yan'an 716000,China;Internal Medicine-Oncology,Shangluo Central Hospital,Shangluo 726000,China;Gastroenterology Ward,Affiliated Hospital of Yan'an University,Yan'an 716000,China)
出处
《实用肿瘤杂志》
CAS
2018年第6期565-569,共5页
Journal of Practical Oncology
