摘要
目的检测自身抗体阳性的免疫性血小板减少症(ITP)患儿CD4^+CD25^+FOXP3^+调节性T细胞(Treg)数量以及自噬相关蛋白的表达。方法通过流式细胞术分选出初诊ITP患儿和健康对照儿童外周血中的Treg,根据抗体的平均荧光强度分别统计自噬相关基因5(ATG5)和ATG7蛋白水平,Western blot法检测CD4^+CD25^+Treg的蛋白激酶B(AKT)和细胞外信号调节激酶(ERK)的磷酸化水平。结果与健康对照儿童相比,ITP患儿Treg数量显著降低,ATG5和ATG7表达降低,AKT和ERK的磷酸化水平均显著降低。结论在初诊自身抗体阳性的ITP患儿中,Treg数量降低,伴随增殖相关蛋白磷酸化水平及细胞自噬水平的降低。
Objective TO detect the number of CD4^+CD25+FOXP3^+regulatory T cells (Tregs)and the expression of autophagy related proteins in autoantibody positive children with immune thrombocytopenia (ITP).Methods Flow cytometry was used to sort Tregs in peripheral blood of newly-diagnosed ITP children or healthy donor controls,the expression of autophagy related gene ATG5or ATG7was calculated by their mean fluorescence intensity,and the phosphorylation of protein kinase B (AKT)or extracellular signal regulatory kinase (ERK)of CD4+CD25^+Tregs was determined by Western blot analysis.Results Compared with healthy donor control,the number of Tregs in ITP children decreased significantly,the expression of ATG5 and ATG7decreased,and the phosphorylation of AKT and ERK decreased significantly.Conclusion In newly-diagnosed auto-antibody positive ITP children,the number of Tregs is reduced,and the levels of protein phosphorylation related to the proliferation and autophagy of Tregs is decreased.
作者
王琦
计雪强
邵惠江
季正华
朱宏
邵雪君
WANG Qi;JI Xueqiang;SHAO Huijiang;JI Zhenghua;ZHU Hong;SHAO Xuejun(Department of Clinical Laboratory,Children's Hospital of Soochow University,Suzhou 215025,China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2018年第9期834-838,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
苏州卫计委民生科技医疗卫生应用基础研究项目(SYSD2017094)
