摘要
介绍了“分子元素”概念。论述了核酸适体的筛选及其作为大分子药物的应用价值,概述了人工碱基的合成及进展;探讨了脱氧核酶的作用原理,分析了分子信标、分子马达在生物传感、生物合成、生物药物研究等方面的应用前景,展望了核酸分子研究领域的前景及面临的挑战。
We put forward an influential concept of "molecular elements"to appreciate the composition of nucleic acids from a new perspeetive.The nucleic acicls that exhibit different functions are designed based on the functional requirement.They are constructed by phosphodiester bomls formation from various "molecular elements"that have different bases,thus achieving a variety of functions such as specific molecular recognition,catalysis,and intelligent response.In this article we present an overview of representative research results of our laboratory upon functional nucleic acid molecules,including the screening and application of nucleic acid aptamers as maeromolecular medicine;the synthesis and evolution of artificial bases;the mechanism of DNAzymes;molecular beacons,molecular motors and their applications in biosensing,biosynthesis,hinpharmaceutieal research.Moreover,we discuss the challenges and future research directions in the field of functionalized nucleic acid.
作者
谭燕
李颖颖
玄文静
王若文
王雪强
谭蔚泓
TAN Yan;LI Yingying;XUAN Wenjing;WANG Ruowen;WANG Xueqiang;TAN Weihong(Molecular Science and Biomedicine Laboratory,College of Chemistry and Chemical Engineering,Hunan University,State Key Laboratory for Chemo/Bio Sensing and Chemometrics,Changsha 410082,China;Institute of Molecular Medicine,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China)
出处
《科技导报》
CAS
CSCD
北大核心
2018年第22期54-65,共12页
Science & Technology Review
基金
国家自然科学基金项目(81602499)
关键词
分子元素
功能核酸
人工碱基
核酸适体
DNA酶
molecular element
functional nucleic acid
artificial base
aptalner
DNAzyme
