摘要
目的评价氢对脓毒症相关性脑病小鼠血脑屏障的影响。方法成年雄性ICR小鼠100只,6~8周龄,体重20~25g,采用随机数字表法分为4组(n=25):假手术组(Sham组)、假手术+氢组(Sham+H组)、脓毒症相关性脑病组(SAE组)和脓毒症相关性脑病+氢组(SAE+H组)。采用盲肠结扎穿孔(CLP)法制备小鼠脓毒症模型。Sham+H组和SAE+H组分别于CLP后l和6h时吸人2%氢气1h。于CLP后24h时尾静脉注射伊文氏蓝(EB),取脑组织,测定EB含量和水含量;光镜下观察海马病理学结果;采用Western blot法测定海马occludin和血管内皮钙粘连蛋白(VE-cadherin)的表达。于CLP后10~16d时行Morris水迷宫实验。结果与Sham组比较,SAE组及SAE+H组脑组织EB含量和水含量升高,海马occludin和VE-cadherin表达下调,逃避潜伏期延长,穿越原平台次数减少(P<0.05)。与SAE组比较,SAE+H组脑组织EB含量和水含量降低,海马occludin和VE-cadherin表达上调,逃避潜伏期缩短,穿越原平台次数增加(P<0.05),海马病理学损伤减轻。结论氢减轻小鼠脓毒症相关性脑病的机制可能与减轻血脑屏障损伤有关。
Objective To evaluate the effect of hydrogen on blood brain barrier of mice with sepsisassociated encephalopathy (SAE).Methods A total of 100adult male ICR mice,aged 6-8weeks, weighing 20-25g,were divided into 4groups (n=25each)using a random number table method:sham operation group (group Sham),sham operation plus hydrogen group (group Sham+H),group SAE and SAE plus hydrogen group (group SAE +H).Sepsis was induced by cecal ligation and puncture (CLP ). Sham+H and SAE+H groups inhaled 2%hydrogen for 1h starting from 1and 6h after CLP,respectively. At 24h after CLP,Evans blue (EB)was injected via the caudal vein,and then the mice were sacrificed and brain tissues were removed for measuring the EB and water contents,for examining the pathological changes of hippocampi (with a light microscope)and for detecting the expression of occludin and VE-cadherin (by Western blot).Morris water maze test was performed at days 10-16after CLP.Results Compared with group Sham,the contents of EB and water in brain tissues were significantly increased,the expression of occludin and VE-cadherin was down-regulated,the escape latency was prolonged,and the number of crossing the original platform was reduced in SAE and SAE+H groups (P<0.05).Compared with group SAE,the contents of EB and water in brain tissues were significantly decreased,the expression of occludin and VE-cadherin was up-regulated,the escape latency was shortened,the number of crossing the original platform was increased (P<0.05),and the pathological changes of hippocampi were significantly attenuated in group SAE+H.Conclusion The mechanism by which hydrogen mitigates SAE may be relatedto reducing the damage to blood brain barrier of mice.
作者
冯竞成
于洋
毛幸
闫梦莹
王国林
谢克亮
于泳浩
Feng Jingcheng;Yu Yang;Mao Xing;Yan Mengying;Wang Guolin;Xie Keliang;Yu Yonghao(Department of Anesthesiology,Tianjin Medical University General Hospital Tianjin Research Institute of Anesthesiology,Tianjin 300052,China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2018年第6期719-722,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金(81671888,81772043)
天津市自然科学基金(13JCQNJC11400).
关键词
氢
脓毒症
脑
血脑屏障
Hydrogen
Sepsis
Brain
Blood-brain barrier
