摘要
目的建立一种骨内微血管可视化及形态学研究的新模型,测量血管参数并对模型可靠性进行评估。方法10只新西兰大白兔行腹主动脉microfill灌注,取股骨远端标本进行固定、脱钙,利用microCT扫描三维重建对骨内微血管进行可视化研究,选取股骨远端标本制成厚约300μm的硬组织切片,在荧光背景下观察骨内微血管分布及形态特征,对血管主要参数进行测量。结果标本脱钙后进行microCT扫描重建,能够屏蔽骨组织CT值与灌注液CT值相近引起的干扰;厚切片荧光背景显像可以特异性显示微血管在骨组织中的分布及形态;在单位面积血管数、平均血管直径、血管面积分数测量中,microCT与组织学之间差异无统计学意义(P=0.075、0.921、0.661),但组织学切片中可视最小血管直径达毛细血管级别,显著小于microCT测量结果[(9.03±1.14)μm比(12.61±2.01)μm,P=0.038]。结论血管灌注、microCT扫描三维重建结合厚切片荧光背景显像可作为一种骨内微血管可视化及形态学研究的可靠方法。
Objective To explore a new method for visualization and morphologic study of intraosseous microvessels, measure the main parameters of the vesselsand evaluate the reliability of this model. Methods Abdominal aorta microfill perfusion in 10 New Zealand white rabbits were used. The specimens of the lower femur were fixed and decalcified sequentially, then the blood vessels were analyzed by microCT scan and three-dimensional reconstruction. The microvessels distribution and morphology of the bone were observed under the background of fluorescence by making the specimen into slices with a thickness of 300 μm. The main parameters of blood vessels were measured and evaluated. Results The structures of the intraosseous microvessels was clearly reconstructed in three dimensions by decalcification which can reduce the interference of the CT value of bone tissue to the CT value of the perfusion fluid. The distribution and morphology of the microvessels in the bone tissue were well displayed in the thick tissue slices in the fluorescence background imaging. There was no statistical difference in the number of blood vessels, mean vessel diameter and vascular percentage of the unit area between the results of microCT and histology (P=0.075, 0.921, 0.661). In histological Histology, the visual minimum blood vessel diameter reached the level of capillaries, significantly less than the results of microCT [(9.03±1.14) μm vs. (12.61±2.01) μm, P=0.038]. Conclusion MicroCT scan three-dimensional reconstruction combined with thick slice fluorescence background imaging can be used as a reliable method for the visualization and morphological study of intraosseous microvessels.
作者
鲁亚杰
李明辉
龙作尧
张浩强
肖鑫
吴杰
王臻
Lu Yajie;Li Minghui;Long Zuoyao;Zhang Haoqiang;Xiao Xin;Wu Jie;Wang Zhen(Department of Orthopaedics,Xijing Hospital,the Air Force MilitaryMedical University,Xi'an 710032,China;Department of Orthopaedics,Lanzhou General Hospital of the People's Liberation Army,Lanzhou 730050,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2018年第12期2336-2338,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(31370944)。
