摘要
目的探讨人三磷酸胞嘧啶脱氧核苷酸(dCTP)焦磷酸酶1在前列腺癌组织和前列腺癌细胞株LNCaP中的表达,观察dCTP焦磷酸酶1的过表达对LNCaP细胞周期的影响。方法免疫组织化学法分析前列腺癌及非癌组织中dCTP焦磷酸酶1的表达量;通过Westernblot检测LNCaP的dCTP焦磷酸酶1表达量(LNCaP:1.26±0.13比RWPE-1:0.52±0.01,P=0.000)。构建dCTP焦磷酸酶1过表达的LNCaP细胞株,并通过流式细胞仪检测LNCaP细胞株细胞周期的变化(LNCaP-dCTP焦磷酸酶1过表达组:48.23±0.66比LNCaP-空载体组:30.10±1.01,P=0.022)。结果dCTP焦磷酸酶1在癌组织中表达上调[免疫组织化学评分:非癌组:(2.00±1.55)分,前列腺癌组:(4.00±1.52)分,P=0.000],dCTP焦磷酸酶1的高表达增加了LNCaP细胞的S+G2期的比例(P=0.000)。结论前列腺癌中的dCTP焦磷酸酶1表达增加,其高表达可能会促进前列腺癌细胞分裂。
Objective To investigate the expression of human deoxycytidine triphosphate pyrophosphatase 1 (dCTP pyrophosphatase 1) in prostate cancer tissues and LNCaP cells, and observe the effect of high expression of dCTP pyrophosphatase 1 on LNCaP cell cycle. Methods The expression of dCTP pyrophosphatase 1 in prostate cancer and non-cancer tissue was analyzed by immunohistochemistry. The expression of dCTP pyrophosphatase 1 protein in LNCaP and RWPE-1 cells was detected by Western blotting (LNCaP: 1.26±0.13 vs. RWPE-1: 0.52±0.01, P=0.000). The dCTP pyrophosphatase 1 overexpressed LNCaP cell lines were constructed and cell cycle changes were detected by flow cytometry (LNCaP-dCTP pyrophosphatase 1: 48.23±0.66 vs. LNCaP-NC: 30.10±1.01, P=0.022). Results The expression of dCTP pyrophosphatase 1 in cancer tissues was up-regulated (IHC score: non-cancer: 2.00±1.55, cancer: 4.00±1.52, P=0.000), and the high expression of dCTP pyrophosphatase 1 increased the S+ G2 phase of LNCaP cells (P=0.000). Conclusion High expression of dCTP pyrophosphatase 1 in prostate cancer promotes the division of prostate cancer cells.
作者
叶永康
米其武
曾彦茹
卢剑铭
钟惟德
Ye Yongkang;Mi Qiwu;Zeng Yanru;Lu Jianming;Zhong Weide(Department of Urology,Dongguan People's Hospital Affiliated to Southern Medical University,Dongguan 523059,China;Department of Urology,Guangzhou First People's Hospital,the Second Affiliated Hospital of South China University of Technology,Key Laboratory of Clinical Molecular Medicine and Molecular Diagnosis,Guangzhou 510180,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2018年第12期2190-2192,共3页
Chinese Journal of Experimental Surgery
基金
广州市科技计划项目(201707010291、201803040001)
广州市卫生计生科技一般引导项目(20171A011239)
广东省科技计划项目(2016A020215018).
