摘要
OBJECTIVE: To investigate the effective ingredients of Danshen(Radix Salviae Miltiorrhizae) and Honghua(Flos Carthami)(Danhong) on protective properties towards neonatal rat hippocampal neurons under hypoxia condition.METHODS: Primary culture of neonatal rat hippocampal neurons was used to model hypoxia damage on the hippocampus. Methyl thiazolyl tetrazolium(MTT) assay and preliminary experiments were conducted to identify the four effective ingredients of Danhong, along with the injection of positive drug onto hippocampal neurons at a non-toxic dosage level. The cultured cells were randomly divided into 12 groups: the normal group, model group,positive drug control group and nine compatibility groups of the four effective ingredients. Different test methods were applied to determine lactate dehydrogenasein(LDH), total superoxide dismutase(T-SOD), malondialdehyde(MDA), 6-keto prostaglandin F_(la)(6-keto-PGF_(1 a)), thromboxane B_2(TXB_2),mitochondrial membrane potential(MMP), free calcium ions concentration([Ca^(2 +)]i) and early onsetcell apoptosis.RESULTS: Different compatibility groups could inhibit the content of LDH and intracellular calcium overload, increase activity in T-SOD, decrease level of MDA and TXB2, improve level of 6-keto-PGF1 aand MMP, and prevent the early onset cell apoptosis.CONCLUSION: The compatibility of four effective ingredients of Danhong had protective effect toward hippocampi hypoxia. The mechanism might be related to inhibit oxidative stress damage and cell apoptosis, resist thrombosis, and reduce the intracellular calcium ion of overload.
OBJECTIVE: To investigate the effective ingredients of Danshen(Radix Salviae Miltiorrhizae) and Honghua(Flos Carthami)(Danhong) on protective properties towards neonatal rat hippocampal neurons under hypoxia condition.METHODS: Primary culture of neonatal rat hippocampal neurons was used to model hypoxia damage on the hippocampus. Methyl thiazolyl tetrazolium(MTT) assay and preliminary experiments were conducted to identify the four effective ingredients of Danhong, along with the injection of positive drug onto hippocampal neurons at a non-toxic dosage level. The cultured cells were randomly divided into 12 groups: the normal group, model group,positive drug control group and nine compatibility groups of the four effective ingredients. Different test methods were applied to determine lactate dehydrogenasein(LDH), total superoxide dismutase(T-SOD), malondialdehyde(MDA), 6-keto prostaglandin F_(la)(6-keto-PGF_(1 a)), thromboxane B_2(TXB_2),mitochondrial membrane potential(MMP), free calcium ions concentration([Ca^(2 +)]i) and early onsetcell apoptosis.RESULTS: Different compatibility groups could inhibit the content of LDH and intracellular calcium overload, increase activity in T-SOD, decrease level of MDA and TXB2, improve level of 6-keto-PGF1 aand MMP, and prevent the early onset cell apoptosis.CONCLUSION: The compatibility of four effective ingredients of Danhong had protective effect toward hippocampi hypoxia. The mechanism might be related to inhibit oxidative stress damage and cell apoptosis, resist thrombosis, and reduce the intracellular calcium ion of overload.
基金
Supported by National Natural Science Foundation of China:Biological Foundation Research Related to Ischemic Stroke in Qi Deficiency and Blood Stasis Syndrome Based on Drug Metabolism and Biological Effects of Buyang Huanwu Decotion and Its Associated Prescriptions(No.81630105)
Effect of Danhong Injection on Cerebral Ischemia Reperfusion Injury in Rats Mediated by Aldose Reductase(No.81673639)
Zhejiang Provincial Natural Science Foundation of China:Mechanism of the Component Compatibility of Salvia Miltiorrhiza and Safflower against Cerebral Ischemic Injury Based on the Association of PK-PD(No.LZ17H270001)
Zhejiang Provincial Program for the health leading talents
Basic public welfare research project of Zhejiang Province:Investigation and Application of Optimization of Chinese Traditional Medicine Extraction Process Based on Genetic Algorithm-neural Network Approach(No.LGN18A010001)
Scientific Research Foundation of Zhejiang Chinese Medicine University:Mechanism of Danhong Injection against Cerebral Ischemic Injury Based on Pharmacokinetic Efficacy(PK-PD)Relationship(No.2017ZZ09)
