摘要
目的通过基因芯片技术进行全基因组拷贝数变异(CNV)分析,筛查与食管鳞状细胞癌(ESCC)放射敏感相关性的基因。方法 选择2013年12月至2016年8月安阳市肿瘤医院放疗科接受单纯放疗且该院病理中心留存有活组织检查石蜡标本的ESCC患者,分为放疗敏感组和放疗抗拒组。对两组患者的石蜡标本进行DNA提取,利用美国Affymetrix公司的OncoScan Array平台设计的基因芯片检测人全基因组CNV,筛选两组样本的基因片段差异。结果共收集到19例ESCC患者的标本进行DNA提取,为方便配对分析,从DNA样品质检合格的患者中选取10例,其中放疗敏感组和放疗抗拒组各5例。两组患者性别、年龄、病变部位、病变长度、放疗剂量差异均无统计学意义(均P>0.05).杂合性缺失(LOH)是主要的CNV类型。放疗抗拒组中,10号染色体q24.32-q24.33区域与18号染色体q21.2-q21.31区域检测到LOH的频率为100%,而在放疗敏感组中未检测到。放疗敏感组中,4号染色体q27-q28.1区域检测到LOH的频率为80%,而在放疗抗拒组中未检测到。结论10q/18q LOH与ESCC放射抗拒相关,4q LOH与ESCC放射敏感相关。
Objective To analyze copy number variance (CNV) in whole genome by using gene chip technology, and to screen the radiosensitivity associated genes on esophageal squamous cell carcinoma (ESCC). Methods The patients with ESCC who received radiotherapy alone in Anyang Tumor Hospital from December 2013 to August 2016 were selected, and biopsy paraffin samples were preserved in the center of pathology. The patients were divided into radiosensitivity group (group S) and radio-resistance group (group R). DNA was extracted from these paraffin samples in both groups. Whole human genome CNV was detected by using genechip from OncoScan Array platform designed by Affymetrix company, and the differences of gene segments were screened in the two groups. Results Nineteen samples of ESCC patients were collected to extract DNA in this study. To balance pair analysis in the two groups, 10 samples were selected from the qualified patients, including 5 cases in group S and 5 cases in group R respectively. There were no statistical differences in gender, age, lesion site, lesion length, radiation dose of the two groups (all P> 0.05). Loss of heterozygosity (LOH) was the main type of CNV. The analysis results showed that LOH in q24.32-q24.33 of chromosome 10 and LOH in q21.2-q21.31 of chromosome 18 had high frequencies (100 %) in group R, however, none were detected in group S. LOH in q27-q28.1 of chromosome 4 had a high frequency (80%) in group S , however, none were detected in group R. Conclusion LOH in 10q/18q is related to radio-resistance in ESCC, and LOH in 4p is associated with radiosensitivity in ESCC.
作者
朱青山
刘维鹏
代宁涛
冯连杰
马庭炜
魏涛
李军扩
韩朋
Zhu Qingshan;Liu Weipeng;Dai Ningtao;Feng Lianjie;Ma Tingwei;Wei Tao;Li Junkuo;Han Peng(Department of Radiotherapy,Anyang Tumor Hospital,Anyang 455000,China;Center of Pathology,Anyang Tumor Hospital,Anyang 455000,China)
出处
《肿瘤研究与临床》
CAS
2018年第11期725-728,738,共5页
Cancer Research and Clinic
基金
河南省安阳市科技攻关计划(201617).
关键词
食管肿瘤
癌
鳞状细胞
基因测定
辐射耐受性
Esophageal neoplasms
Carcinoma,squamous cell
Genetic testing
Radiation tolerance
