摘要
目的探究心宝丸治疗慢性心力衰竭(CHF)的多成分、多靶点、多通路的作用机制。方法根据Cardiovascular Disease Herbal Database(CVDHD)数据库检索心宝丸中药材的有效成分,利用Cytoscape 3.6.1软件建立"成分-靶点"网络,通过Kyoto Encyclopedia of Genes and Genomes(KEGG)数据库进行筛选靶点的相关通路分析,最后利用CVDHD数据库构建心宝丸"成分-靶点-通路-疾病"网络分析心宝丸对其他疾病的潜在作用。结果预测实验表明,心宝丸中活性成分29个,作用靶点约为11个,共涉及通路24条,其中,心宝丸抗CHF的靶蛋白主要通过Cyclic Adenosine monophosphate(cAMP)信号通路、肾素分泌通路、 Cyclicguanosine monophosphate(c GMP)-Protein kinase G(PKG)信号通路、Toll样受体信号通路、Mitogen-activatedprotein kinases(MAPK)信号通路、多巴胺能突触通路、雌激素信号通路、Rap1信号通路等8条通路,调节心血管炎症过程、参与心肌重塑、增强心肌收缩力,调节心肌氧化代谢和激素水平发挥抗CHF的作用。心宝丸还对高血压、肥厚型心肌病、扩张型心肌病、Ⅱ型糖尿病、冠状动脉疾病、深静脉血栓形成等疾病具有潜在治疗作用。结论调节心血管炎症过程、参与心肌重塑、增强心肌收缩力,调节心肌氧化代谢和激素水平可能是心宝丸发挥抗CHF的作用机制之一。
Objective This study was designed to explore the multi-component,multi-target and multi-pathway mechanisms of Xinbao Pills(XBP)in the treatment of chronic heart failure(CHF).Methods The component-target network was constructed using Cytoscape 3.6.1 software based on the active molecules of the XBP extracted from the Cardiovascular Disease Herbal Database (CVDHD).The obtained targets were analyzed and screened using the Kyoto Encyclopedia of Genes and Genomes (KEGG)database.A network map of component-target-pathwaydisease was also constructed to analyze the potential effects of XBP on other diseases. Results It showed that 29 main active constituents of XBP and 11 relate targets involved 24 pathways,8 of which included Cyclic adenosine monophosphate (cAMP) signaling pathway,renin secretion pathway,Cyclic guanosine monophosphate (cGMP)-Protein kinase G(PKG)signaling pathway,Toll-like receptor signaling pathway,Mitogen-activated protein kinases(MAPK) signaling pathway, dopaminergic synaptic pathway, estrogen signaling pathway and Rap1 signaling pathway. They may be closely related to the CHF involving in the regulation of cardiovascular inflammatory processes, participation in myocardial remodeling,enhancement of myocardial contractility,myocardial oxidative metabolism and hormone levels. In addition, XBP had potential therapeutic effects on hypertension, hypertrophic cardiomyopathy,dilated cardiomyopathy,type 2 diabetes,coronary artery disease,deep vein thrombosis and so forth. Conclusion Regulation of cardiovascular inflammatory process,myocardial oxidative metabolism and hormone levels as well as participation in myocardial remodeling, enhancement of myocardial contractility may be the mechanisms of XBP exerting anti-chronic heart failure effect.
作者
李尊江
于潇潇
王冬梅
古江勇
刘云涛
丁邦晗
吴晓新
LI Zunjiang;YU Xiaoxiao;WANG Dongmei;GU Jiangyong;LIU Yuntao;DING Banghan;WU Xiaoxin(Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou 510120 Guangdong,China;The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120 Guangdong,China;Key Laboratory of Traditional Chinese Medicine Emergency Research,Guangzhou 510120 Guangdong,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2018年第6期768-774,共7页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省中医药局名优中成药二次开发项目(20170410)
