摘要
目的研究血塞通分散片对慢性脑缺血大鼠模型的治疗作用,并通过检测前列环素I_2(PGI_2)/血栓素A_2(TXA_2)及一氧化氮(NO)/血管内皮舒张因子(EDRF)信号通路的变化,探讨其作用机制。方法采用永久性双侧颈总动脉结扎法建立慢性脑缺血大鼠模型。将SD大鼠随机分为模型组,假手术组,灯盏细辛颗粒组(1.5 g·kg^(-1)),血塞通分散片高、中、低剂量组(50,25,12.5 mg·kg^(-1)),每组10只,每天灌胃给药1次,连续8周。采用酶联免疫吸附实验检测血清细胞间黏附分子-1(ICAM-1)、PGI_2、TXA_2、NO、EDRF含量;分别称定脑组织湿质量、干质量,计算脑组织含水量。结果与假手术组比较,模型组大鼠的脑组织含水量及血清炎症因子ICAM-1含量均明显增加(P <0.01),血清PGI_2、NO、EDRF含量明显降低(P <0.05,P <0.01),TXA_2含量明显增加(P <0.05)。与模型组比较,血塞通分散片高、中剂量组能明显降低慢性脑缺血大鼠脑组织的含水量和血清ICAM-1、TXA_2含量(P <0.05, P <0.01),明显增加血清PGI_2、NO、EDRF含量(P <0.05,P <0.01)。结论血塞通分散片可能通过降低炎症因子ICAM-1、调节PGI2/TXA2及NO/EDRF信号通路,发挥对慢性脑缺血的治疗作用。
Objective To study the effect of Xuesaitong dispersion tablets on brain edema and inflammation caused by chronic cerebral ischemia in the rat model;and determine the influence of Xuesaitong dispersion tablets on PGI2/ TXA2 and EDRF/NO signaling pathways to further investigate the mechanism. Methods Chronic cerebral ischemia rat models caused by permanent bilateral common carotid artery ligation. The SD rats were randomly devided into model group,Sham group,Dengzhan Xixin granule group (1.5 g·kg^-1 ) and Xuesaitong high,medium and low dose groups (50,25,12.5 mg·kg^-1 ,respectively),10 rats in each group,and administrated with correspondent drugs once a day for 10 days. Serum contents of ICAM-1,PGI2,TXA2,NO,EDRF were detected using ELISA;and the fresh weight and dry weight of brain tissue were measured to calculate the water content of brain tissue. Results Compared with Sham group rats,water content of brain tissue and the serum levels of ICAM-1 and TXA2 in model rats were significantly increased (P<0.01), the serum contents of PGI2, NO and EDRF were significantly decreased (P<0.05,P<0.01). Compared with model rats,water content of brain tissue and the serum levels of ICAM-1 and TXA2 were significantly decreased in the high and medium dose Xuesaitong dispersion tablets group rats (P<0.05,P<0.01),and the serum contents of PGI2,NO and EDRF were significantly increased (P<0.05, P<0.01). Conclusion Xuesaitong dispersion tablets has a beneficial effect on chronic cerebral ischemia via regulating PGI2/TXA2 and EDRF/NO signaling pathways.
作者
吕小波
张明泽
黄春球
梅艳
昂骏英
武正才
LYU Xiaobo;ZHANG Mingze;HUANG Chunqiu;MEI Yan;ANG Junying;WU Zhengcai(Department of Research and Development,Yunnan Phytopharmaceutical CO.,LTD,Kunming 650109 Yunnan,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2018年第6期698-701,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
云南省科技厅生物医药重大科技专项(2016RA036)
