摘要
目的探讨术前结直肠癌患者与健康对照组外周血T/B/NK淋巴细胞亚群绝对数的差别,及术后奥沙利铂、卡培他滨化疗对结直肠癌患者外周血T/B/NK淋巴细胞亚群的影响。方法应用流式细胞仪体积法,对2017年5月至2018年1月就诊于唐山市人民医院的86例结直肠癌住院患者,在其化疗前后分别进行外周血T/B/NK淋巴细胞亚群检测,同期对健康对照组外周血T/B/NK淋巴细胞亚群进行检测,应用SPSS 24.0统计学软件对所收集数据进行统计学分析。结果 (1)术前结直肠癌患者与健康对照组外周血CD3^+、CD3^+CD4^+、CD3^+CD8^+、CD3^-CD19^+、CD16^+CD56^+细胞绝对数比较,差异具有统计学意义(P <0. 001);结直肠癌患者CD3^+CD4^+/CD3^+CD8^+、CD3^-CD19^+和CD16^+CD56^+水平与患病年龄、性别、肿瘤定位无关;与TNM分期相关。(2)经奥沙利铂、卡培他滨治疗后结直肠癌患者外周血CD3^+、CD3^+CD4^+、CD3^-CD19^+、CD16^+CD56^+细胞绝对数较化疗前明显降低,差异具有统计学意义(P <0.001);而CD3^+CD8^+细胞绝对数较化疗前明显升高,差异具有统计学意义(t=4.167,P<0.001)。结论外周血T/B/NK淋巴细胞亚群水平与结直肠癌密切相关;结直肠癌患者经奥沙利铂、卡培他滨治疗后外周血T/B/NK淋巴细胞亚群的绝对数明显下降,其细胞免疫、体液免疫和自然杀伤细胞的功能显著降低。
Objective To explore the difference of the absolute numbers of T/B/NK lymphocyte subsets between preoperative colorectal cancer patients and healthy controls,and the effect of oxaliplatin and capecitabine chemotherapy on T/B/NK lymphocyte subsets in peripheral blood of patients with colorectal cancer after the operation.Methods The flow cytometry was used to examine 86 cases of colorectal cancer hospitalized in Tangshan City people's Hospital from May 2017 to January 2018.The peripheral blood T/B/NK lymphocyte subsets were detected before and after the chemotherapy.The T/B/NK lymphocyte subsets in the peripheral blood of the healthy control group were detected at the same time,and the SPSS24.0statistics software was applied for the statistical analysis of the collected data.Results (1)The absolute numbers of CD3^+,CD3^+CD4^+,CD3^+CD8^+,CD3^-CD19^+ and CD16^+CD56^+ cells in the peripheral blood of colorectal cancer patients and the healthy control group were significantly different (P <0.001).The levels of CD3^+CD4^+/CD3^+CD8^+,CD3^-CD19^+ and CD16^+CD56^+ in colorectal cancer patients were not related with the age,sex,and tumor location,but correlated with TNM staging.(2)The absolute numbers of CD3^+,CD3^+ CD4^+,CD3^-CD19^+ and CD16^+CD56^+ cells in peripheral blood decreased significantly in patients with colorectal cancer after the oxaliplatin and capecitabine,(P <0.001),and the absolute number of CD3^+CD8^+ cells was significantly higher than that of pre-chemotherapy(t=4.167,P <0.001).Conclusion The levels of T/B/NK lymphocyte subsets in peripheral blood are closely related with colorectal cancer;the absolute numbers of T/B/NK lymphocyte subsets in peripheral blood of colorectal cancer patients decrease significantly after the oxaliplatin and capecitabine,and the function of cell immunity,humoral immunity and natural killer cells decrease significantly.
作者
李世龙
张宝
宋杨
张海光
秦莉
吴丹
苗雅静
LI Shi-long;ZHANG Bao;SONG Yang;ZHANG Hai-guang;QIN Li;WU Dan;MIAO Ya-jing(Tangshan Municipal people's Hospital,Tangshan,Hebei Province,Tangshan 063020,China;Department of Clinical Laboratory,Tangshan Hospital for Infectious Diseases,Hebei Province,Tangshan 063020,China;Luanxian people's Hospital of Tangshan City,Hebei Province,Luanxian 063700,China)
出处
《标记免疫分析与临床》
CAS
2018年第11期1607-1610,共4页
Labeled Immunoassays and Clinical Medicine
基金
河北省卫生和计划生育委员会科研基金项目(编号:20181234)
