摘要
目的:发展一种通过三维形态学参数评价阿司匹林抑制血小板聚集行为的新方法。方法:将采集的12名健康志愿者枸橼酸钠抗凝外周血均分为二份,一份加入200μmol/L乙酰水杨酸(ASA)处理作为实验组,另一份为对照组,分别对两组血小板进行洗涤纯化。将纯化血小板加入Ⅰ型胶原蛋白修饰的反应池中在静态条件下诱导血小板活化聚集,通过形状测量激光显微系统观察分析形成的血小板聚集体三维形态。同时,通过光透射浊度法(LTA)分析血小板功能。结果:本技术可在无标记情况下获取血小板聚集体的形貌、高度及三维形态图像数据,并可量化血小板聚集体的体积参数; ASA处理能明显改变血小板聚集体的三维形态学特征;与对照组相比,实验组血小板聚集体体积显著降低(t=8. 97,P <0. 01),而横截面积改变不显著(t=1. 94,P> 0. 05)。受试者工作特征曲线(ROC)分析结果显示,血小板聚集体体积截断值为1 395μm3时鉴别ASA抑制功效的灵敏度和特异度分别为91. 7%和75%,其准确度和灵敏度均优于LTA方法测试的聚集度参数。结论:本研究建立的ASA抑制血小板聚集行为的三维形态学分析方法将为后续研究和临床评价ASA抗血小板聚集效应提供新的分析策略。
Objective: To develop a new method for evaluating the inhibitory effect of aspirin on platelet by the threedimensional ( 3D) morphological parameters.Methods: The sodium citrate-anticoagulant peripheral blood samples collected from 12 healthy volunteers were divided into 2 groups: group treated with 200 μmol /L acetylsalicylic acid ( ASA) ,and control group.The platelets in the 2 groups were washed and purified.The purified platelets were added into reaction pools modified with type I collagen and induced to activation and aggregation under static condition.The 3D morphology of the formed platelet aggregate was measured by the laser shape microscopic system.Meanwhile,the platelet function was detected by turbidometric light transmittance aggregometry ( LTA) .Results: This technology could acquire the shape,height and 3D morphology of the platelet aggregates without label,and could quantify their volume parameters.ASA treatment could obviously change the morphology of platelet aggregates.Compared with the parameters of control group,the volume of platelet aggregates in experimental group decreased significantly ( t = 8.97,P<0.01) ,while the cross-sectional area showed no significant change ( t = 1.94,P>0.05) .The receiver-operating characteristic curve( ROC) analysis showed that the platelet aggregate volume as a parameter to identify the ASA inhibition effect had 91.7% sensitivity and 75% specificity when the cut-off value equal to 1395 μm3 ,and its accuracy and sensitivity were both better than those of platelet aggregates rate measured by LTA method.Conclusion: The new method developed for evaluating the ASA inhibition of platelet aggregation may provide a complementary strategy for researching and clinically evaluating of ASA anti-platelet aggregation in future.
作者
刘娟
丁玲
何翠
陈丹
邓素容
李远
LIU Juan;DING Ling;HE Cui;CHEN Dan;DENG Su-Rong;LI Yuan(Central Laboratory of Yongchuan Hospital,Chongqing Medical University;Yongchuan Sub-center of Chongqing Blood Center,Chongqing 402160,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2018年第6期1757-1764,共8页
Journal of Experimental Hematology
基金
重庆市社会事业与民生保障科技创新专项(cstc2017shmsA130009)
重庆市医学科研计划项目(2017MSXM079)
重庆医科大学附属永川医院院级重点项目(YJZQN201534
YJZQN201533)
关键词
血小板聚集
阿司匹林
三维形态
Ⅰ型胶原蛋白
血小板聚集体体积
platelet aggregation
aspirin
three-dimensional morphology
type Ⅰ collagen
platelet aggregate volume
