摘要
目的以乌药主要化学成分预测其作用靶点和药效作用,构建乌药多成分-多靶点网络。方法选取乌药挥发油、生物碱和呋喃倍半萜及其内酯3大类成分中的7个代表性化合物(乌药烯酯、异瑟模环烯醇、新木姜子碱、β-葎草烯、母菊薁、六驳碱、香樟内酯)为研究对象,利用网络药理学的方法对其潜在作用靶点和通路进行预测,通过数据整合剖析乌药主要化学成分药效作用。结果 7个化合物可作用于40个潜在靶点和20条相关信号通路,涉及抗炎、镇痛、胃肠运动调节、抗氧化、抗肿瘤、肝损伤保护等多个环节;各类成分之间有共同的作用靶点及通路群,有共同的药效作用又各有侧重。结论乌药挥发油、生物碱和呋喃倍半萜及其内酯通过多个蛋白靶点和信号通路的调节与转导,显示出不同成分间的多靶点、多途径的协同作用,为系统研究乌药药效作用及机制提供了参考。
Objective To predict the pharmacological effect and targets of main chemical components of Lindera aggregata,and construct the multi-target network.Methods The potential targets and pathways of linderene acetate,isocembrol,laurolitsine, β-humulene,chamazulene,laurotetanine,and lindestrenolide,selected as the representative compounds of oils,alkaloids,and furan sesquiterpene lactones from Linderae Radix,were predicted by methods of network pharmacology.The pharmacodynamic effects of main chemical constituents of L.aggregata was analyzed by data integration.Results The in silico prediction results showed that seven compounds ofL.aggregata affected 20related pathways through 40potential targets.The pathways were involved in several links including inflammatory,analgesic,gastrointestinal movement regulation,anti-oxidation,antitumor,liver injury protection,and immunoregulation.The various components showed common targets,pathways,and pharmacodynamic effects and had different emphases.Conclusion The pathways related to L.aggregata oils,alkaloids,and furan sesquiterpene lactones were connected by the common targets,showing the synergistic effect of different compounds by acting on multi-targets and multi-pathways.This study provides references for systematic exploration on the pharmacological actions and mechanisms of L.aggregata.
作者
邓桂明
向彪
肖小芹
欧阳林旗
刘景诗
魏凤
朱青
蒋司晨
DENG Gui-ming;XIANG Biao;XIAO Xiao-qin;OUYANG Lin-qi;LILT Jing-shi;WEI Feng;ZHU Qing;JIANG Si-chen(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;The College of Integrated Traditional Chinese and Western medicine of Hunan University of Chinese Medicine,Changsha 410208,China;Department of Pharmacy,The Affiliate Cancer Hospital of Xiangya School of Medicine,Central South University,Changsha 410013,China)
出处
《中草药》
CAS
CSCD
北大核心
2018年第21期5125-5133,共9页
Chinese Traditional and Herbal Drugs
基金
中国博士后基金项目(2016T90752):湖南省中医药科研计划项目(201429)
湖南省教育厅项目(14A107)
2017年湖南省重点中医建设项目(湘中医药医函[2017]11号)
张志国全国名老中医药专家传承工作室建设项目(国中医药人教教育[2016])
湖南省自然科学基金资助项目(2018JJ6040).
关键词
乌药
网络药理学
挥发油
生物碱
呋喃倍半萜及其内酯
乌药烯酯
异瑟模环烯醇
新木姜子碱
β-葎草烯
母菊薁
六驳碱
香樟内酯
Lindera aggregata (Sims)Kosterm
network pharmacology
.volatile oil
alkaloid
sesquiterpene lactones
linderene acetate
isocembrol
laurolitsine
β-humulene
chamazulene
laurotetanine,lindestrenolide
