摘要
目的 比较编码流感病毒A/PR/8/34（H1N1）蛋白的各类DNA疫苗在BALB/c 小鼠的免疫原性。方法 分别将编码流感病毒血凝素（hemagglutinin，HA）、神经氨酸酶（neuraminidase，NA）、基质蛋白（matrix protein，M1）、核蛋白（nucleoprotein，NP）以及非结构蛋白（nonstructural protein，NSI）基因克隆入表达质粒。用基因枪轰击法将构建的重组DNA转入小鼠表皮，共2次，间隔3周。第2次免疫7d后，用同源病毒攻击小鼠，通过检测小鼠肺部病毒量和小鼠存活率来评价上述各类DNA对小鼠的免疫原性。结果HA、NA DNA能诱导小鼠产生高滴度的特异性抗体，并提供有效的保护。而 M1、NP、NS1 DNA不能提供有效的保护，尽管其中用 M1、NP DNA免疫的小鼠能检测到抗体应答。结论 病毒表面糖蛋白HA、NA DNA具有较强的免疫原性。
Abstract: Objective To compare the immunogenicities of various DNAs encoding influenza virus A/ PR/8/34(HlNl) protein in BALB/c mice. Methods Recombinant DNAs were constructed by cloning the genes encoding hemagglutinin (HA), neuraminidas (NA), matrix protein (M1), nucleoprotein, (NP) and nonstructural (NS1) into expression vector pCAGGSP7 respectively and inoculated to BALB/c mice by gene gun twice at an interval of 3 weeks. The mice were challenged with the homologous viruses 7 days after the second dose, and the lung virus liters and survival rate of them were detected and used for the evaluation of the protections induced by the DNAs. Results Specific antibodies at high levels and good protection were induced in the mice immunized with HA- and NA-expressing DNAs. Ml- and NP-expressing DNAs showed antibody responses but no good protection. However, no antibody response was detected out in the mice immunized with NSl-expressing DNA. Conclusion Both HA- and NA-expressing DNAs showed the strongest protections against influenza among the 5 kinds of DNAs.
出处
《中国生物制品学杂志》
CAS
CSCD
2002年第1期13-16,共4页
Chinese Journal of Biologicals
